Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Assigning taxonomy to shotgun metagenomic reads: sequence alignment or composition?

    I'm interested in identifying bacterial pathogens from shotgun metagenomic data. Crudely, I envisage the pipeline to be something like this:
    1) QC reads
    2) Filter out human sequences
    3) Identify pathogen sequences

    Typically it seems people use the following types of tools for the corresponding steps above:
    1) Trimmomatic/cut-adapt
    2) Bowtie2/SNAP/BWA against human reference genome(s)
    3) Bowtie2/SNAP/BWA against reference bacterial genomes database

    My question is can steps 2) and/or 3) be replaced with a sequence composition based approach? (E.g. a k-mer method, such as Kraken, LMAT or CLARK)

    Of course k-mer methods will be faster but less sensitive than read alignment with a tool like Bowtie2. Additionally, read alignment uses paired-read and quality score info that k-mer approaches don't use. However, there seems to be an increasing trend to adopt 'alignment-free' or 'psudoalignment' approaches and I'm not sure how to evaluate the trade-offs. I'd be grateful for any advice. Thanks.

  • #2
    A recent analysis of HMP data used a k-mer approach (LMAT) to remove additional human variation:
    An international, peer-reviewed genome sciences journal featuring outstanding original research that offers novel insights into the biology of all organisms

    Comment


    • #3
      Yes I don't think LMAT is preferable to Kraken though; since it has a much larger memory footprint. However, this dosen't address the more fundamental question of whether k-mer approaches are preferable to read alignment or not

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Latest Developments in Precision Medicine
        by seqadmin



        Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

        Somatic Genomics
        “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
        05-24-2024, 01:16 PM
      • seqadmin
        Recent Advances in Sequencing Analysis Tools
        by seqadmin


        The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
        05-06-2024, 07:48 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 05-24-2024, 07:15 AM
      0 responses
      193 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-23-2024, 10:28 AM
      0 responses
      212 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-23-2024, 07:35 AM
      0 responses
      213 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-22-2024, 02:06 PM
      0 responses
      12 views
      0 likes
      Last Post seqadmin  
      Working...
      X