targeted sequencing... in microbiology

[molgene]

Hi all,

I'm working on bacteria.
I received the full sequence of a microrganism. The genome was 9Mb in length.

I performed several tests on this microrganisms (they took 2 years) and now we want to know if these tests were responsible for changes in a specific region of the genome (0.2Mb).

I'm a newbie in sequencing so I'd like to ask if the targeted resequencing could be useful for me!

What do you think?

Additional question:
I read this sentence on Illumina website:
"Regions of interest are captured by hybridization to biotinylated probes and then isolated by magnetic pulldown. Target enrichment captures 20 kb–62 Mb regions, depending on the experimental design."
but I didn't find a lots of paper using this technique on bacteria genomes... so is there something that I'm not considering about this technique that does not allow me to use it for my purpose?

I'd really appreciate any help.
Thanks

[nucacidhunter]

One of the reasons for targeted capture sequencing is to lower the cost of sequencing. This makes sense for species with large genomes. Bacteria genomes are small and sequencing whole genome is cheaper than sequence capture.

[molgene]

yes you're totally right. Thanks for reply.

To be sincere I don't know exactly how much will be my targeted sequencing, but I thought that testing microrganisms after every test would decrease the cost. But probably I'm wrong.

[GenoMax]

Targeted sequencing may make sense only if you need to process hundreds (or thousands) of samples and are only interested in a narrow region.

[molgene]

Targeted sequencing may make sense only if you need to process hundreds (or thousands) of samples and are only interested in a narrow region.

Ok thanks!