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  • aabi
    Member
    • May 2016
    • 35

    Advanced RNA-Seq Analysis Training @NIH, USA, Jan 10-13, 2017

    Hands-on Training in Advanced RNA-Seq Analysis
    Jan 10-13, 2017

    Where?
    National Institutes of Health
    9000 Rockville Pike
    Building 60, Room 162
    Bethesda, MD 20892

    Objectives


    Participants will start with learning to use two different suite of programs (Tuxedo Vs Trinity) and compare their results for differential expression analysis. Participants will then learn advanced RNA-Seq analysis techniques and tools for detecting snps, fusion genes, allele specific expressions, circular RNAs,viral/bacterial sequence identification, alternative polyadenylation and transcriptional regulatorynetwork analysis.

    Hands-on Skills/Tools Taught

    Differential expression analysis : Tuxedo tools
    Differential expression analysis : Trinity tools
    Fusion gene analysis : Tophat-Fusion
    SNP detection : GATK
    Allele specific expression : WASP
    Circular RNA identification : circexplorer
    Alternative polyadenylation : DaPars
    Viral integration : VirusFinder
    Network analysis : Cytoscape

    Highlights
    • Participants will work with a Graphic User Interface based Linux Desktop environment in the Amazon Cloud, that is specially configured to run popular open source RNA-Seq analysis tools.
    • Participants will be provided with free access to the fully configured Amazon Machine Image for their personal use after the training.
    • Participants will also receive a cookbook style manual for all the handson exercises.
    • After training support is also provided through exclusive members only forum.
    • Hands-on training provided by NIH researchers active in the field of RNA-Seq analysis

    For more information and registration, please visit the following page;
    Information and Registration

Latest Articles

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    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.


    Here are nine questions we think about, in roughly the order they matter, before...
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  • SEQadmin2
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    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
    ...
    06-02-2026, 10:05 AM

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