Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • deepbiomed
    Junior Member
    • Apr 2013
    • 8

    #1

    STAR aligner - Segmentation fault (core dumped) multiple fastq paired-end samples

    Hi,
    I am using STAR-2.5.2b on virtualbox with 65 gb of RAM assigned and 28 CPUs/threads.
    When I ran STAR with single paired-end sample, it worked like a charm, very quick compared to TopHat but when I am trying to run STAR with multiple samples, I got the core dumped error.

    1) VirtualBox:/media/sf_John/star/CR004/test_together_,$ '/media/sf_John/star/STAR-2.5.2b/bin/Linux_x86_64/STAR' --runThreadN 28 --sjdbOverhang 99 --genomeDir '/media/sf_John/star/genome_build' --readFilesIn '/media/sf_John/star/CR004/samples/CR004_S24_L005_R1_001.fastq', '/media/sf_John/star/CR004/samples/CR004_S24_L005_R2_001.fastq' '/media/sf_John/star/CR004/samples/CR004_S24_L006_R1_001.fastq', '/media/sf_John/star/CR004/samples/CR004_S24_L006_R2_001.fastq' --outFileNamePrefix SampleA --chimSegmentMin 20

    Segmentation fault (core dumped)

    also tried

    2) VirtualBox:/media/sf_John/star/CR004/test_together_,$ '/media/sf_John/star/STAR-2.5.2b/bin/Linux_x86_64/STAR' --sjdbOverhang 99 --genomeDir '/media/sf_John/star/genome_build' --readFilesIn /media/sf_John/star/CR004/samples/*.fastq --outFileNamePrefix SampleA --chimSegmentMin 20

    Segmentation fault (core dumped)

    same error. I know this has to do something with memory but don't know how to resolve it.

    Please guide.
    Thanks
    D
  • wdecoster
    Member
    • Oct 2015
    • 97

    #2
    Cross posted

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2





      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
      10-30-2026, 06:46 AM
    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 11-05-2026, 09:25 AM
    0 responses
    20 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-03-2026, 11:54 AM
    0 responses
    34 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    82 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-28-2026, 09:51 AM
    0 responses
    50 views
    0 reactions
    Last Post SEQadmin2  
    Working...