I have a need to assign dispersed individuals back to a clutch of eggs. I have many samples from each clutch of eggs. Rather than conducting a sibship analysis between the eggs and the dispersed adults, would it be possible to homogenize a batch of eggs and sequence them as one sample that approximates the parental genomes and then conduct a parentage analysis to assign individuals back to their clutch?
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For reference cross-posted: https://www.biostars.org/p/221930/
If is ok to cross-post on SeqAnswers.
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Sounds like a reasonable approach to me. The clutch presumably has paternal and maternal alleles, while a single individual will have half of the alleles present in the clutch, but I think the relationship would be clear unless the different clutches are highly similar.Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com
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