Error in maker (gene prediction)

[rajesh gazara]

Hello Everyone,

Does anyone know about below error in maker annotation :
ERROR: Non-unique top level ID
While this is technically legal in GFF3, it usually
indicates a poorly fomatted GFF3 file (perhaps you
tried to merge two GFF3 files without accounting for
unique IDs). MAKER will not handle these correctly.

I used fasta file only with one contig. I don't know why I am getting Non-unique ID error.

If anyone know please reply. I am waiting for responses.
Thanking you in advance!

Regards,
Rajesh

[colindaven]

It seems you have included a GFF3 file in your maker options - one of your conf files. I would recommend supplying evidence as FASTA files to annotate your genome with, so make sure all the GFF3 files in the options are commented out.

[rajesh gazara]

Hi colindaven,

Thanks for your reply. I made it.