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Setup sequence aligner in MPICH2 cluster
I am really new to cluster computer but I want to try it because using 1 computer take a bit of time. In my lab, I have several old PC so I think it would be good if I have a cluster computer. I think I will follow MPICH2 cluster in Ubuntu because it looks simple. My question is, can Bowtie2/BWA aligner be used from MPICH2 cluster? How to do it? Thank you.
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You can actually try Rocks instead. Very few programs are truly parallel in NGS data analysis area so MPICH2 is not going to help.
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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