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Hi Everyone,
I have been having some issues with my last few runs and was hoping someone on here might be able to help out. I routinely do low diversity sequencing so I am very familiar with matrix workarounds ( high phi x, dark reads, etc). However the last 5 or so runs the first 7 bases have had almost the exact same raw intensities and then separate starting at round 8. The images are from a 90% phi x run. When I open the matrix file It is very obvious that the matrix that it is setting is incorrect. In the corrected intensity the T drops to zero for the remainder of the run after the erroneous matrix is set. I set the density low on this one(800) for a V3 kit. I have also tried the same thing on a V2 nanokit and while the raw intensities still looked the same as this one (all bunch for the first few cycles) the matrix was calculated correctly and it was able to generate accurate FastQs. Has anyone else seen anything like this?
I have been having some issues with my last few runs and was hoping someone on here might be able to help out. I routinely do low diversity sequencing so I am very familiar with matrix workarounds ( high phi x, dark reads, etc). However the last 5 or so runs the first 7 bases have had almost the exact same raw intensities and then separate starting at round 8. The images are from a 90% phi x run. When I open the matrix file It is very obvious that the matrix that it is setting is incorrect. In the corrected intensity the T drops to zero for the remainder of the run after the erroneous matrix is set. I set the density low on this one(800) for a V3 kit. I have also tried the same thing on a V2 nanokit and while the raw intensities still looked the same as this one (all bunch for the first few cycles) the matrix was calculated correctly and it was able to generate accurate FastQs. Has anyone else seen anything like this?
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