Howdy,
Tried to post this once, and it disappeared.
I have been tasked with assembling a per genome cost of Sequel sequencing (assuming we own the instrument already). We are primarily looking to close microbial genomes in the 2 - 8 Mbp range.
I read 5-8 Gbp of output from a single Sequel run, and I'm also hearing we can multiplex 2 genomes per run pretty safely - seems to be we could do more, but maybe there's something I'm not seeing about the math or the actual "usable" percentage of the 5 Gbp estimated output... or it is a gross overestimate when used in the real world.
I've heard $1000 / SMRT cell, but that doesn't include all the reagents necessary to make libraries, barcode, etc. Excluding the cost of DNA extraction (unless it is for some reason more expensive than Illumina/ONT), any help or insight that could be provided on running multiple microbial genomes on a single Sequel SMRT cell, primarily from a cost basis, would be greatly appreciated.
Best,
DocBio
Tried to post this once, and it disappeared.
I have been tasked with assembling a per genome cost of Sequel sequencing (assuming we own the instrument already). We are primarily looking to close microbial genomes in the 2 - 8 Mbp range.
I read 5-8 Gbp of output from a single Sequel run, and I'm also hearing we can multiplex 2 genomes per run pretty safely - seems to be we could do more, but maybe there's something I'm not seeing about the math or the actual "usable" percentage of the 5 Gbp estimated output... or it is a gross overestimate when used in the real world.
I've heard $1000 / SMRT cell, but that doesn't include all the reagents necessary to make libraries, barcode, etc. Excluding the cost of DNA extraction (unless it is for some reason more expensive than Illumina/ONT), any help or insight that could be provided on running multiple microbial genomes on a single Sequel SMRT cell, primarily from a cost basis, would be greatly appreciated.
Best,
DocBio
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