Tweet
MVE Protocols?
Does anyone have MVE protocols?
Does anyone have MVE protocols?
-
-
About number of samples
Hi all,
I need informations,
What's the maximum number of samples that I could put inside a 454 GS Junior's microtiter plate ?
ThanksComment
-
is there any protocol for library preparation when transcriptome sequencing using 454?
thx
Comment
-
i will use 454 for transcriptome sequencing of ploids
so if anybody have done such work please give me some suggestions
thank you very much
EMAIL: [email protected]Comment
-
Expired adaptors
Has anyone used adaptors with expired date for 454 rapid library preparation? I've bought the adaptors (up to 10 samples) but I used them for just 2 DNA samples, now I have 8 more samples for library prep and the adaptors date just expired. I don't know if I try to use them or if I buy new ones.Comment
-
If they've just recently expired, they'd still work and you'll be okay.Comment
-
Thanks RCJK and Eric.Comment
-
hi
i also need a copy of the 454 Titanium Sequencing Manual please? could you kindly send it to me. It will be a great help for me.
email: [email protected]Comment
-
-
NGS data analysis
Thank u so much for the manual.......
can anyone tell me how much time (approx) does it take to analyse the NGS generated data..Comment
-
hi
can anyone tell me how much time does it take to analyse data generated by NGS? I am doin PhD, so just curious to know?Comment
-
We use a 454 GS Junior and have used expired library prep reagents, and I know of other groups that have used them. We have had problems with library preps both with and without expired reagents so I can't say for sure that it will work, but it is certainly worth a shot. Our technical rep suggested a PCR that amps from the adapters--this will tell you if the adapters have successfully ligated and also to check for the approximate size range of the fragments (since we don't have a bioanalyzer). This would let you know whether it is worth pursuing emPCR rather than going through and not getting any enriched beads (we have found that out the hard way). Let me know if you want the details on the PCR.Originally posted by LFMar View PostComment
-
Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...07-09-2026, 11:10 AM -
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...07-08-2026, 05:17 AM -
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
Comment