Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • glacierbird
    Member
    • Dec 2009
    • 15

    samtools view error: CIGAR and sequence length are inconsistent (tophat/bowtie)

    Hi,
    When I try to use "samtools view" to creat .bam file from .sam, I get these error msg. The .sam file is created by mapping raw illumina reads with tophat.

    samtools view -bS -t index.fa.fai -o accepted_hits.bam accepted_hits.sam
    The tag [ID] required for [PG] not present.
    [sam_header_read2] 7409 sequences loaded.
    Parse error at line 8263: CIGAR and sequence length are inconsistent

    This #line 8263 is like this:

    head -n 8263 accepted_hits.sam |tail -n 1
    HWI-EAS210R:5:55:1018:1930#0 0 SL1.00sc00002 428427 0 36M92N36M473N536870911M * 0 0 CTCATCTCTCATCTCAGAGAGGTCCTCCAGGAGCAGGAATATAAGTTGGATCAAGTACGCAATCATCTTCA XXQZXR^Ta\[a\ZVZ^ZYXWWYPSUMSSQXZTO^ZZPV_\`_U\VYY[W^G\SJSTTRTUTXFTTQTYVS NM:i:0 XS:A:+ NS:i:0

    Anybody could explain me why Samtools gave such error msg?
    Thanks!
  • glacierbird
    Member
    • Dec 2009
    • 15

    #2
    I think the problem is caused by:

    36M92N36M473N536870911M

    But, I don't know how could it occur?

    Comment

    • glacierbird
      Member
      • Dec 2009
      • 15

      #3
      I think the solution could be:
      reformat the raw .fastq file.

      Maybe, somewhere is not Tab, but space.

      then, 536870911M
      will not occur.

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
        by SEQadmin2


        Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

        The systematic characterization of the human proteome has
        ...
        07-20-2026, 11:48 AM
      • SEQadmin2
        Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
        by SEQadmin2



        Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
        ...
        07-09-2026, 11:10 AM
      • SEQadmin2
        Cancer Drug Resistance: The Lingering Barrier to Rising Survival
        by SEQadmin2



        Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

        There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
        07-08-2026, 05:17 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, Yesterday, 12:17 PM
      0 responses
      13 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-23-2026, 11:41 AM
      0 responses
      13 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-20-2026, 11:10 AM
      0 responses
      23 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-13-2026, 10:26 AM
      0 responses
      37 views
      0 reactions
      Last Post SEQadmin2  
      Working...