Tweet
Hello, my ddRAD libraries (mean 550 pb) contain the first 5 identical bases at their ends, belonging to the partial restriction site. They will be paired-end sequenced in a MiSeq. I would like to modify the Illumina sequencing primers by adding those 5 bases at the 3 prime end of each primer. Thus, a higher diversity is expected in the first sequenced bases and also an adequate cluster generation. Does anyone have experience with this approach? and the use or not, and its PhiX concentration. Thank you. Best regards.
-
-
It could work - however you will not be able to sue the PhiX libraries as spike in since you will can't use the Illumina sequencing primers together with your extended versions. -
Thank you. It is right. So the question is what can work best. 1) Use modified primers to bypass the restriction site or 2) use a high concentration of PhiX (how much?).Comment
-
Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.
Somatic Genomics
“We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...05-24-2024, 01:16 PM -
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM
Comment