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RAD-Seq Library Loading Concentration?
Hello,
Would I be correct in assuming that RAD-Seq libraries should be treated as if they were low-diversity amplicon libraries (i.e. 16S rDNA/microbiome) for the purposes of loading? In other words, we typically load amplicons at 4.0 pM with a 10% PhiX spike-in - would the best practices be the same for the RAD-Seq libraries?
Thanks...
Would I be correct in assuming that RAD-Seq libraries should be treated as if they were low-diversity amplicon libraries (i.e. 16S rDNA/microbiome) for the purposes of loading? In other words, we typically load amplicons at 4.0 pM with a 10% PhiX spike-in - would the best practices be the same for the RAD-Seq libraries?
Thanks...
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