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Hi,
This may be a naive question. We are new to SNP discovery using sequencing. We have designed custom AmpliSeq DNA sequencing panel to look for germline gene polymorphism for one gene using human PBMCs. We wonder if controls are needed for this type of experiments. For example, to include a reference sample with known SNPs? But it will be impossible to include positive controls for every possible genotype, right?
Thank you for your opinions and advice in advance.
This may be a naive question. We are new to SNP discovery using sequencing. We have designed custom AmpliSeq DNA sequencing panel to look for germline gene polymorphism for one gene using human PBMCs. We wonder if controls are needed for this type of experiments. For example, to include a reference sample with known SNPs? But it will be impossible to include positive controls for every possible genotype, right?
Thank you for your opinions and advice in advance.