Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • neolith
    Junior Member
    • May 2009
    • 3

    #1

    Mosaik Aligning with 454 Reads

    How long will it take to finish the Mosaik Aligning work?

    In the manual for Mosaik1.0, MosaikAligner aligned the lane of 6,563,762 Illumina paired-end reads(36bp) in 45.9minutes (2,390 paired-end reads/s) to the entire human genome(2.9Gbp) using 8processor cores.

    And in the manual for Mosaik, Aligner setting for 454 GS-FLX : -hs 15 -mm 0.05 -act 26

    But in the case of version 0.9.891, the lane of 119,263 '454 reads'
    expected time of alignment over 50 hours.(0.6 reads/s)

    Used commands are :
    MosaikAligner -in input.dat -out aligned.dat -ia total.ref.dat -hs 15 -mm 0.05 -act 26 -m all -mhp 100 -j h.sapiens_15 -p 8

    Mosaik version : 0.9.891-linux-x64

    I will do the job with version 1.0.1367, and I want to reduce working hour...
  • neolith
    Junior Member
    • May 2009
    • 3

    #2
    In the case of Mosaik version 1.0.1367,
    aligned the lane of 119,263 '454 reads', in 246minutes (7.7 reads/s)
    to the entire human genome(3.0Gbp) using 8 processor cores....

    Comment

    • donniemarco
      Member
      • Aug 2009
      • 17

      #3
      How can I create one single dat example total.ref.dat for the entire genome. The data available are in seperate files for each chromosome.
      I want to run entire index data with mosaik for all the chromosome together.
      Thanks!!

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
        by SEQadmin2



        CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

        Despite this, “CRISPR helped turn genome editing from a specialized technique into
        ...
        07-31-2026, 11:01 AM
      • SEQadmin2
        Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
        by SEQadmin2


        Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

        The systematic characterization of the human proteome has
        ...
        07-20-2026, 11:48 AM
      • SEQadmin2
        Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
        by SEQadmin2



        Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
        ...
        07-09-2026, 11:10 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 08-03-2026, 10:13 AM
      0 responses
      15 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-31-2026, 02:55 AM
      0 responses
      32 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-24-2026, 12:17 PM
      0 responses
      23 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-23-2026, 11:41 AM
      0 responses
      21 views
      0 reactions
      Last Post SEQadmin2  
      Working...