Has anyone here used the Opentrons OT-2 for library preparation? https://opentrons.com/ot-2
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"Accuracy Accurate down to 384-well plates"
This is a new measure of accuracy that I was previously unaware of.
Their only 'Sequencing Application' in their protocol library is for 16S. There are liquid handling platforms with a much wider array of NGS protocols ready to go than this.. -
A neighboring lab has the OT-One. This first version is unusable for just about anything - it can't hold the pipettes properly and the plates have a lot of play. Version two looks very different, though.Comment
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There have recently been a few discussions about them on Reddit; perhaps it might be a way to get in touch with people who use them.Comment
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We recently got an OT2. So far it works for single channel pipetting.
We have problems with both of our multi-channel pipettes.
The magnetic module seems to have a design flaw - the magnet is too close to the samples, even when disengaged and at maximum distance (achieved with an self-made adapter). Thus, the beads do not stay in suspension for long - ideally they should stay in suspension for 5 minutes before then engaging the magnet.Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...Yesterday, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM -
Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...07-09-2026, 11:10 AM
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