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  • beyg
    Junior Member
    • May 2019
    • 1
    #1

    How to get better alignment results of PAcBio data?

    HI. I am trying to align PAcBio data (data from third generation sequencer) using bwa mem. I am getting very less number of aligned reads in SAM file having coverage of less than 10X. I tried to use minimap2 as well for alignment but again the quality of SAMfile isn,t good. I am getting only few unique reads and they are aligning on reference with soft clipping. Moreover, my SAM file contains many cases of multimapping. The average read length of spots is >3kb.
    What other options are available to get better alignment results? I am working with this data for the very first time.
    Tags: bwa mem, pacbio alignment, sam file
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