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Hi, my name is Alexandre Bueno. I installed consed ( from phredPhrap package) on two machines. One of them is an old pc and the other is a notebook. Both machines have Xubuntu 18.04 64bit installed in them. The consed version running on the computers is 20.0. the executable of consed is consedlinux64bit_static. After running phredPhrap succesfully, I execute the consed program. The main window of the software opens up. Unfortunetely when I try, file > export consensus with options, it's not possible to type any digits in the field.None of any keyboard's input are recognized by the software and the fields remain blank. I am also sending an image of the problem.The problem happened in both machines. I need help fixing this .Thank you in advance.
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM