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I'm using unique dual index primer pairs for my experiments. I'm multiplexing around 12 samples per NextSeq run. I don't know how to create a custom library in BaseSpace that will allow me to assign the unique primer pairs to all my samples as it's limited by a 12 x 8 format (12- i7 indexes and 8-i5 indexes, I need to be able to enter 12 i5 indexes). Any help would be greatly appreciated.
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I ran into a similar issue recently. If you have the Dx version of the NextSeq, there is a software update that can do FASTQ generation on the machine similar to the MiSeq.
The other option is a work around where you will have to create two custom library kit. Each kit can do 8 UDI samples if you set your samples in a diagonal so that each row and column will have an unique index. After that just pool them together in the pool tab in BaseSpace. -
I don't have the Dx version but your workaround will be just fine, I didn't think it would let me combine samples from two different "kits". Thanks for the help!Comment
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custom library
Here's the bulletin from Illumina describing how to create a custom library prep kit in BaseSpace.Comment
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The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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