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Hey NextSeq500 users, has anyone experienced an unexpected power failure during their run? Have you determined any causes? A instrument power failure occurred 10 hours into my last run.
Tracy
Tracy
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As in the sequencer just died while the power was still on in building? Do you not use UPS's on your sequencers to filter out power anomalies? -
The power failure was not a result of a utility failure. Yes, the instrument was connected to a UPS. It appears to be an instrument power failure.Comment
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I assume you have eliminated a faulty UPS as a possible cause? If your sequencer is under a maintenance contract you should contact Illumina tech support ASAP.
Never seen a sequencer just die (out of 20 over years) so something is not right.Comment
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Update: NextSeq500 Power Failure
The power failure turned out to be a failure of one the computer fans in the instrument. After 10 hours into the run when the computer started heating up with the processing of data, the processing unit overheated and shutdown.Comment
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I get that a lot. Mostly because I have a lot of electronics and a lot of load on the network. Sometimes it goes out. The main thing is to use the equipment well. Because a short circuit can happen. And it can burn out all the wiring, which nobody wants, I'm sure. And that's it, I've got the guys for everything, it's the residential electricians Minneapolis. My main thing is that they solve the problem quickly and efficiently. Everyone's place of residence, of course. But you get the point. I don't have to deal with it myself, I'd rather pay and stay healthy.Last edited by Lymanara; 02-13-2021, 07:15 AM.Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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