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Can somebody help me in getting the new RL protocol compatible with the Nimblegen capture downstream? I tried to design the old Titanium adaptors myself with a T overhang but they didn't work obviously. Got no PCR product. Has somebody ever tried that approach successfully? Perhaps I made a mistake during oligo/adaptor design??? Thanks so much. Tom
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I think the primers you would normally use for you LM-PCR work on the rapid library as well. The biggest problem I see is that your blockers (Hybridization Enhancing Oligo's) won't work as well as they do for general libraries so if this would effect your ontarget you'll have to design them. Perhaps even per MID.
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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