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Illumina is going to re-introduce ribozero equivalent kits in 2020.
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For those seeking a bacterial rRNA depletion kit, NEB has just released a kit that works very well in our hands.
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DISCLAIMER: I work for ZymoOriginally posted by cement_head View Post
We have options for people who are interested in doing probe-free depletion. Contact [email protected] and tell them you'd like to try the RiboFree Total RNA Library Kit. We were offering trial sizes and free samples a few months ago.Comment
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According to rumors, RiboZero will make a comeback. Not exactly the same products though; it will use a new chemistry.Comment
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Illumina did release a new version of RiboZero+ yesterday: https://www.illumina.com/products/by...depletion.htmlComment
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Interesting. Appears to use a strand-denaturation/hybridization-based normalization to remove/reduce concentrations of highly represented cDNAs. That is, enzymatic digestion of DNA/RNA duplexes that form due to C0t kinetics.Originally posted by jjlin View Post
This would make it species agnostic. Specified to work down to 100ng or somewhat below that with extended hybridization times.
Most of the charm of the old Truseq Ribozero-depletion library kits was that although they specified a limited range of species, eg "human/mouse/rats" the probes were very long (not oligos) and so actually seemed to work on any animal. For example they worked on moth RNA in our hands.
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PhillipComment
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Thanks. No manual available yet. List price is $82 per reaction.
Originally posted by GenoMax View PostComment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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