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Hi GA/Hiseq users!,
I'm trouble with cluster problem.
One is concetration determination of library. I always use real-time PCR with serial diluted library and PhiX standard.
Because library is serially diluted, so molar concentration of library is also determined with various molar conc range. I thought this way wolud provide reliable cluster density. However result was bad. Although suitable molar concentration of libary based on qRTPCR data, for 350K cluster/tile generation, was used, always clusters were not able to achieve the goal.
If you have any idea that can make reliable cluster density, would you like to let me know?
Thanks.
I'm trouble with cluster problem.
One is concetration determination of library. I always use real-time PCR with serial diluted library and PhiX standard.
Because library is serially diluted, so molar concentration of library is also determined with various molar conc range. I thought this way wolud provide reliable cluster density. However result was bad. Although suitable molar concentration of libary based on qRTPCR data, for 350K cluster/tile generation, was used, always clusters were not able to achieve the goal.
If you have any idea that can make reliable cluster density, would you like to let me know?
Thanks.