Tweet
Is there any website that I can use to download the chromosome 3 of barley?
-
-
You might want to start from here, http://barleygenome.org/ -
Alternatively, you can go straight to the raw reads on NCBI's SRA, run a search "hordeum vulgar [orgn] chromosome 3" and you should come up with the details, this link might work.
If you need more details, I'd recommend looking through this paper, for which the supplementary information contains a list of ordered sequences that you could use to generate the sequence of barley chromosome 3. I don't believe there is yet a resource where you can download an assembly as such, but if you follow the link from the previous post I may be proven wrong.Comment
-
Thanks a lot for your replys, pj_oz the NCBI's link is what I am looking for
thanks
Comment
-
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
Comment