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|02-15-2012, 07:46 PM||#1|
Location: Pittsburgh, PA
Join Date: Nov 2008
Untangling Genomes from Metagenomes
Untangling Genomes from Metagenomes: Revealing an Uncultured Class of Marine Euryarchaeota
Authors: Vaughn Iverson, Robert M. Morris, Christian D. Frazar, Chris T. Berthiaume, Rhonda L. Morales, E. Virginia Armbrust*
School of Oceanography, University of Washington, Box 357940, Seattle, WA 98195, USA.
Ecosystems are shaped by complex communities of mostly unculturable microbes. Metagenomes provide a fragmented view of such communities, but the ecosystem functions of major groups of organisms remain mysterious. To better characterize members of these communities, we developed methods to reconstruct genomes directly from mate-paired short-read metagenomes. We closed a genome representing the as-yet uncultured marine group II Euryarchaeota, assembled de novo from 1.7% of a metagenome sequenced from surface seawater. The genome describes a motile, photo-heterotrophic cell focused on degradation of protein and lipids and clarifies the origin of proteorhodopsin. It also demonstrates that high-coverage mate-paired sequence can overcome assembly difficulties caused by interstrain variation in complex microbial communities, enabling inference of ecosystem functions for uncultured members.
Nature News article about this paper: http://www.nature.com/news/one-genome-from-many-1.9969
|de novo assembly, dna sequencing, metagenomics|