Chip Viewer

[genseq]

Hi,

Which program allows to view pH-sensore chips and to estimate their quality ?

Thanks!

[BBoy]

This is a puzzling statement. The identification of unloaded sites should be relatively straightforward. As for "estimating their quality" it is not clear how that can be done a-priori.

The whole idea of a "chip washing" station makes sense mostly for very low volume use in very price constrained environments. If the (Poisson) chip loading on the first pass is ~50% then you get ~25% on the second pass. And if the washing requires time-consuming quality control after that... you are already behind the ball. Being just an amateur in the field, I do not have hard data on the relative cost of chip and reagents, but I suspect that the latter dwarfs the former. Saving $250 on the cost of chip but maintaining $10k on the cost of reagents while getting only 50% of the data does not strike me as a viable proposal. Am I missing something?

[genseq]

Originally Posted by BBoy

This is a puzzling statement. The identification of unloaded sites should be relatively straightforward. As for "estimating their quality" it is not clear how that can be done a-priori.

The whole idea of a "chip washing" station makes sense mostly for very low volume use in very price constrained environments. If the (Poisson) chip loading on the first pass is ~50% then you get ~25% on the second pass. And if the washing requires time-consuming quality control after that... you are already behind the ball. Being just an amateur in the field, I do not have hard data on the relative cost of chip and reagents, but I suspect that the latter dwarfs the former. Saving $250 on the cost of chip but maintaining $10k on the cost of reagents while getting only 50% of the data does not strike me as a viable proposal. Am I missing something?

Coast of semiconductor sequencing - ~750$.
Coast of chip (Ion 316) - 299$.
You can save ~40%.

[BBoy]

Originally Posted by genseq

Coast of semiconductor sequencing - ~750$.
Coast of chip (Ion 316) - 299$.
You can save ~40%.

40% off the cost of consumables. Much less of the cost of "the answer" In any case, if I read the proposal correctly. The first time they simply use the same chip but just read the freshly loaded sites. Assuming 50% loading efficiency they get 50% of the data the second time around. The third time they start washing, and you have to add the cost of that - both washing as well as requalification. There will be savings in the end, and LifeTech will hate you to boot, but I still don't think that in a high volume situation the savings will be enough to offset the inconvenience and the data risk.

One problem I see is that once a bead is in place it is held by Van der Waals forces. For a 1um size bead the gravitational effects are sufficiently low that it can be difficult to dislodge a bead from a well.

[genseq]

Waste recycling?
This is terrible!