I use FACS sorted leukocytes to carry out ChIP-seq. After crosslinking in formaldehyde the cells become very sticky and difficult to pellet. Instead, they appear to adhere to the Eppendorf tube walls. I seem to loose a lot of cells at this stage. Any ideas how to prevent or overcome the problem?
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
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