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Ion Torrent Suite v3.2 (base call improvements)
old
@D612M:20:48
GAGTGATAGCTGATGCATT
+
%+2//066337<<=888
On reanalyzing with v3.2
@03KHJ:00020:00048
GAGGGGTGGATAGCTGATGCATT
+
=5555)33*277321884422;+
%+2 // 066337<<=888
old GAG---TG-ATAGCTGATGCATT
new GAGGGGTGGATAGCTGATGCATT
=5555)33*277321884422;+
v3.2 removes many short reads which have low quality.
v3.2 adds homopolymers which it must have been undercounting.
@D612M:20:48
GAGTGATAGCTGATGCATT
+
%+2//066337<<=888
On reanalyzing with v3.2
@03KHJ:00020:00048
GAGGGGTGGATAGCTGATGCATT
+
=5555)33*277321884422;+
%+2 // 066337<<=888
old GAG---TG-ATAGCTGATGCATT
new GAGGGGTGGATAGCTGATGCATT
=5555)33*277321884422;+
v3.2 removes many short reads which have low quality.
v3.2 adds homopolymers which it must have been undercounting.