qPCR Library Quantification

[theward]

From reading other threads most people prefer the KAPA kits to run qPCR for library quantification. Can anyone tell me why these kits are superior to the SYBR green kits as my understanding is they're more expensive? If it to do with the standards being normalised could you not just use your own library to generate a standard curve with the SYBR green kits?
Any advice most welcome!

[microgirl123]

We use the KAPA kit for convenience and because we know it works! The standards are premade, the sizing conversion is easy, and I've never had it fail.

[GW_OK]

Same here. It's incredibly easy to use and always gives reproducible results.

[theward]

Can I ask if you are still sizing the libraries with the bioanalyser/tapestation? If not, how are you sizing the libraries?

[GW_OK]

Tapestation for size and initial quantification in the ballpark range. Kapa kit for actual dilution calculations.

[kwaraska]

Same for us. We use tapestation for quant and size to normalize so it ends up on our standard curve.