[aniacoco]

Please contact me if you have a bad review for the epMotion 5075

We have an epMotion 5075, and it is awful. You have very little control over the machine, the software is sophmoric. It wastes tips, can't be customized, ependorf won't let you make your own labware definitions so you're hostage to their 1 employee that does it, you have no access to the coding language to fix their poor software routines, customer support sucks. Don't buy

I just found your post about the epMotion 5075. I see it was two years ago but if you are still around would you be able to contact me to discuss your review further. I have some questions for you. We are in the process of gathering information on this robot (specifically bad reviews) and your help would be greatly appreciated.

[kzarn]

Are there any updates here regarding good or bad reviews of the epMotion 5075 TMX? Or other models?

Thanks!

[acockburn]

I like the epMotion 5075. I think its a great little robot, especially if you are new to programming these types of devices. I like that it checks to make sure the proper labware is being used and has a lot of fail safes for people who use the device infrequently.

That being said, I do like that the BioMex allows you to define your own labware and you can certainly customize it more then the epMotion, but you have to have the training and time to do that. It does not 'check' to ensure the labware is correct or even present, so I found it to be much more time consuming to learn to program and use.

[meyerti]

Hacking EpMotion

Hi,
we have a epMotion 5070 PC and an older 5075 control panel.
True, they are terribly unflexible. With some trial and error one can define own labware but it's more or less undocumented. We have our own 3D printed plates and had to go through this...
But I still could not find a way to define my own methods, or better, to control the machine with matlab instead of epBlue. E.g. i need the machine to pipette while moving.

Does anyone have experience on that?

Best

Tim

[ShyN]

I know this is an old thread however - update!

We've had pretty bad luck with the EP Motion - we use it for Illumina TruSeq library prep and we had anywhere from 40%-80% rRNA contamination when using the machine as well as adapter dimers.
We've since switched over to a hybrid protocol and have been doing the ribo-depletion manually which sucks because it is very time costly.

We've been working with Eppendorf to fix the protocols but haven't had much success..

If anyone has had this experience and has found a way to fix it, advice would be much appreciated!

[BrianJames]

Hi ShyN,
What ribo depletion are you using? We've had good luck with the NEB reagent, and it is a pretty easy protocol. We've been using the EP motion for our polyA libraries with the NEB Ultra II directional kit (1/2 reactions) with very good results. We do some steps off deck, but all of the cleanups are on the instrument.
Hope that helps.
Brian

[cement_head]

Hi,
We ran the EP exclusively for our Illumina Lib preps as well as qPCR set up. Some tweeks were needed regarding labware definitions and aspiration speeds/depths but once we worked out the kinks we fell in love.

Is there a way for the end-user to manually tweak the labware definition files, and/or create new ones, or does on have to entirely rely on Eppendorf for those files?

[cement_head]

Hi,
we have a epMotion 5070 PC and an older 5075 control panel.
True, they are terribly unflexible. With some trial and error one can define own labware but it's more or less undocumented. We have our own 3D printed plates and had to go through this...
But I still could not find a way to define my own methods, or better, to control the machine with matlab instead of epBlue. E.g. i need the machine to pipette while moving.

Does anyone have experience on that?

Best

Tim

I'd be very interested in how you managed to define your own labware - can you post a guide and/or PM me? TIA