Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    too small output .sai file created by bwa aln? ..leads to bwa sampe hanging?

    I have sequenced a 6MB region of a single chromosome of the dog genome (targeted capture).

    At first I used a fasta file from GenBank of the particular chromosome as a reference sequence to map my reads. I indexed the ref sequence and ran bwa aln, which created a .sai file of about 330 MB for each read. I subsequently ran bwa sampe, which succesfully generated a 9.7 GB .sam file from the paired-end reads.

    After this I was recommended to map my reads to the complete genome. So I downloaded the following fasta files from ensemble: each chromosome (1-38), MT, and non-chromosomal DNA and merged the files by cat chr1 chr2 chr3...> mergedFasta.fa. I indexed the fasta file by bwa index and then I ran bwa aln to create .sai files. I got no error messages but noticed that the output file now was 60MB (compared to the 330 .sai file I got when mapping to the single chr GenBank file). I tried to run bwa sampe on the generated .sai files but the program is hanging within 2 minutes. I assume this has to do with the initial creation of the .sai file and the reference FASTA file, but I cant figure out what is wrong? Can somebody help me please?
    Tags: None
  • #2
    It might be your merged fa file. Are you running on 32 bit or 64 bit machine? How long does it take to INDEX? Do you still have the output of the index step? How many files generated and what's their sizes? Can you show the *.ann file here?

    Comment

    • #3
      Hi,

      Thank you so much for trying to help me.

      1) I run Mac OS X 64-bit and bwa is compiled in 64-bit

      2) Indexing of my fasta file (bwa index my.fasta) takes about 50 min.

      3) Output from indexing:
      [bwa_index] Pack FASTA... 38.71 sec
      [bwa_index] Construct BWT for the packed sequence...
      [BWTIncCreate] textLength=4821953750, availableWord=351290412
      [BWTIncConstructFromPacked] 10 iterations done. 99999990 characters processed.
      [BWTIncConstructFromPacked] 20 iterations done. 199999990 characters processed.
      [BWTIncConstructFromPacked] 30 iterations done. 299999990 characters processed.
      [BWTIncConstructFromPacked] 40 iterations done. 399999990 characters processed.
      [BWTIncConstructFromPacked] 50 iterations done. 499999990 characters processed.
      ...
      ...
      [BWTIncConstructFromPacked] 540 iterations done. 4800883702 characters processed.
      [BWTIncConstructFromPacked] 550 iterations done. 4821953750 characters processed.
      [bwt_gen] Finished constructing BWT in 550 iterations.
      [bwa_index] 2105.34 seconds elapse.
      [bwa_index] Update BWT... 50.61 sec
      [bwa_index] Pack forward-only FASTA... 28.83 sec
      [bwa_index] Construct SA from BWT and Occ... 159.11 sec
      [main] Version: 0.6.2-r126
      [main] CMD: bwa index refGen3.fasta
      [main] Real time: 2781.934 sec; CPU: 2382.606 sec

      3) generated files from indexing:
      refGen3.fasta.amb 393 KB
      refGen3.fasta.ann 307 KB
      refGen3.fasta.bwt 2.41 GB
      refGen3.fasta.pac 602.7 MB
      refGen3.fasta.sa 1.21 GB

      4) The .ann file is quite long, but here is the start, do you need more?

      2410976875 3268 11
      0 1 dna:chromosome chromosome:CanFam3.1:1:1:122678785:1 REF
      0 122678785 1122
      0 2 dna:chromosome chromosome:CanFam3.1:2:1:85426708:1 REF
      122678785 85426708 828
      0 3 dna:chromosome chromosome:CanFam3.1:3:1:91889043:1 REF
      208105493 91889043 707
      0 4 dna:chromosome chromosome:CanFam3.1:4:1:88276631:1 REF
      299994536 88276631 622
      0 5 dna:chromosome chromosome:CanFam3.1:5:1:88915250:1 REF
      388271167 88915250 892
      0 6 dna:chromosome chromosome:CanFam3.1:6:1:77573801:1 REF
      477186417 77573801 786
      0 7 dna:chromosome chromosome:CanFam3.1:7:1:80974532:1 REF
      554760218 80974532 607
      0 8 dna:chromosome chromosome:CanFam3.1:8:1:74330416:1 REF
      635734750 74330416 583
      0 9 dna:chromosome chromosome:CanFam3.1:9:1:61074082:1 REF
      710065166 61074082 764
      0 10 dna:chromosome chromosome:CanFam3.1:10:1:69331447:1 REF
      771139248 69331447 744
      0 11 dna:chromosome chromosome:CanFam3.1:11:1:74389097:1 REF
      840470695 74389097 533
      0 12 dna:chromosome chromosome:CanFam3.1:12:1:72498081:1 REF
      914859792 72498081 482
      0 13 dna:chromosome chromosome:CanFam3.1:13:1:63241923:1 REF
      987357873 63241923 488
      0 14 dna:chromosome chromosome:CanFam3.1:14:1:60966679:1 REF
      1050599796 60966679 345
      0 15 dna:chromosome chromosome:CanFam3.1:15:1:64190966:1 REF
      1111566475 64190966 637
      0 16 dna:chromosome chromosome:CanFam3.1:16:1:59632846:1 REF
      1175757441 59632846 442
      0 17 dna:chromosome chromosome:CanFam3.1:17:1:64289059:1 REF
      1235390287 64289059 513
      0 18 dna:chromosome chromosome:CanFam3.1:18:1:55844845:1 REF
      1299679346 55844845 589
      0 19 dna:chromosome chromosome:CanFam3.1:19:1:53741614:1 REF
      1355524191 53741614 304
      0 20 dna:chromosome chromosome:CanFam3.1:20:1:58134056:1 REF
      1409265805 58134056 687
      0 21 dna:chromosome chromosome:CanFam3.1:21:1:50858623:1 REF
      1467399861 50858623 374
      0 22 dna:chromosome chromosome:CanFam3.1:22:1:61439934:1 REF
      1518258484 61439934 371
      0 23 dna:chromosome chromosome:CanFam3.1:23:1:52294480:1 REF
      1579698418 52294480 308
      0 24 dna:chromosome chromosome:CanFam3.1:24:1:47698779:1 REF
      1631992898 47698779 455
      0 25 dna:chromosome chromosome:CanFam3.1:25:1:51628933:1 REF
      1679691677 51628933 384
      0 26 dna:chromosome chromosome:CanFam3.1:26:1:38964690:1 REF
      1731320610 38964690 391
      0 27 dna:chromosome chromosome:CanFam3.1:27:1:45876710:1 REF
      1770285300 45876710 393
      0 28 dna:chromosome chromosome:CanFam3.1:28:1:41182112:1 REF
      1816162010 41182112 395
      0 29 dna:chromosome chromosome:CanFam3.1:29:1:41845238:1 REF
      1857344122 41845238 264
      0 30 dna:chromosome chromosome:CanFam3.1:30:1:40214260:1 REF
      1899189360 40214260 372
      0 31 dna:chromosome chromosome:CanFam3.1:31:1:39895921:1 REF
      1939403620 39895921 288
      0 32 dna:chromosome chromosome:CanFam3.1:32:1:38810281:1 REF
      1979299541 38810281 190
      0 33 dna:chromosome chromosome:CanFam3.1:33:1:31377067:1 REF
      2018109822 31377067 264
      0 34 dna:chromosome chromosome:CanFam3.1:34:1:42124431:1 REF
      2049486889 42124431 297
      0 35 dna:chromosome chromosome:CanFam3.1:35:1:26524999:1 REF
      2091611320 26524999 211
      0 36 dna:chromosome chromosome:CanFam3.1:36:1:30810995:1 REF
      2118136319 30810995 254
      0 37 dna:chromosome chromosome:CanFam3.1:37:1:30902991:1 REF
      2148947314 30902991 327
      0 38 dna:chromosome chromosome:CanFam3.1:38:1:23914537:1 REF
      2179850305 23914537 308
      0 X dna:chromosome chromosome:CanFam3.1:X:1:123869142:1 REF
      2203764842 123869142 1032
      0 MT dna:chromosome chromosome:CanFam3.1:MT:1:16727:1 REF
      2327633984 16727 0
      0 AAEX03026072.1 dna:scaffold scaffold:CanFam3.1:AAEX03026072.1:1:1115:1 REF
      2327650711 1115 0
      0 AAEX03026071.1 dna:scaffold scaffold:CanFam3.1:AAEX03026071.1:1:1573:1 REF
      2327651826 1573 0
      0 AAEX03026070.1 dna:scaffold scaffold:CanFam3.1:AAEX03026070.1:1:1685:1 REF
      2327653399 1685 0
      0 AAEX03026069.1 dna:scaffold scaffold:CanFam3.1:AAEX03026069.1:1:1790:1 REF
      2327655084 1790 0
      0 AAEX03026068.1 dna:scaffold scaffold:CanFam3.1:AAEX03026068.1:1:1819:1 REF
      2327656874 1819 0
      0 AAEX03026067.1 dna:scaffold scaffold:CanFam3.1:AAEX03026067.1:1:1999:1 REF
      2327658693 1999 0
      0 AAEX03026066.1 dna:scaffold scaffold:CanFam3.1:AAEX03026066.1:1:2046:1 REF
      2327660692 2046 0
      0 AAEX03026065.1 dna:scaffold scaffold:CanFam3.1:AAEX03026065.1:1:2145:1 REF
      2327662738 2145 0
      0 AAEX03026064.1 dna:scaffold scaffold:CanFam3.1:AAEX03026064.1:1:2250:1 REF
      2327664883 2250 0
      0 AAEX03026063.1 dna:scaffold scaffold:CanFam3.1:AAEX03026063.1:1:2327:1 REF
      2327667133 2327 0
      0 AAEX03026062.1 dna:scaffold scaffold:CanFam3.1:AAEX03026062.1:1:2361:1 REF
      2327669460 2361 0
      0 AAEX03026061.1 dna:scaffold scaffold:CanFam3.1:AAEX03026061.1:1:2494:1 REF
      2327671821 2494 0
      0 AAEX03026060.1 dna:scaffold scaffold:CanFam3.1:AAEX03026060.1:1:2641:1 REF
      2327674315 2641 0
      0 AAEX03026059.1 dna:scaffold scaffold:CanFam3.1:AAEX03026059.1:1:2700:1 REF
      2327676956 2700 0
      0 AAEX03026058.1 dna:scaffold scaffold:CanFam3.1:AAEX03026058.1:1:2708:1 REF
      2327679656 2708 0
      0 AAEX03026057.1 dna:scaffold scaffold:CanFam3.1:AAEX03026057.1:1:2729:1 REF
      2327682364 2729 0
      0 AAEX03026056.1 dna:scaffold scaffold:CanFam3.1:AAEX03026056.1:1:2772:1 REF
      2327685093 2772 0
      0 AAEX03026055.1 dna:scaffold scaffold:CanFam3.1:AAEX03026055.1:1:2809:1 REF
      2327687865 2809 0
      0 AAEX03026054.1 dna:scaffold scaffold:CanFam3.1:AAEX03026054.1:1:2823:1 REF
      2327690674 2823 0
      0 AAEX03026053.1 dna:scaffold scaffold:CanFam3.1:AAEX03026053.1:1:2850:1 REF
      2327693497 2850 0
      0 AAEX03026052.1 dna:scaffold scaffold:CanFam3.1:AAEX03026052.1:1:2933:1 REF
      2327696347 2933 0
      0 AAEX03026051.1 dna:scaffold scaffold:CanFam3.1:AAEX03026051.1:1:2955:1 REF
      2327699280 2955 0
      0 AAEX03026050.1 dna:scaffold scaffold:CanFam3.1:AAEX03026050.1:1:2997:1 REF
      2327702235 2997 0
      0 AAEX03026049.1 dna:scaffold scaffold:CanFam3.1:AAEX03026049.1:1:3011:1 REF
      2327705232 3011 0
      0 AAEX03026048.1 dna:scaffold scaffold:CanFam3.1:AAEX03026048.1:1:3059:1 REF
      2327708243 3059 0
      0 AAEX03026047.1 dna:scaffold scaffold:CanFam3.1:AAEX03026047.1:1:3134:1 REF
      2327711302 3134 0
      0 AAEX03026046.1 dna:scaffold scaffold:CanFam3.1:AAEX03026046.1:1:3135:1 REF
      2327714436 3135 0
      0 AAEX03026045.1 dna:scaffold scaffold:CanFam3.1:AAEX03026045.1:1:3231:1 REF
      2327717571 3231 0
      0 AAEX03026043.1 dna:scaffold scaffold:CanFam3.1:AAEX03026043.1:1:3257:1 REF
      2327720802 3257 0
      0 AAEX03026044.1 dna:scaffold scaffold:CanFam3.1:AAEX03026044.1:1:3257:1 REF
      2327724059 3257 0
      0 AAEX03026042.1 dna:scaffold scaffold:CanFam3.1:AAEX03026042.1:1:3271:1 REF
      2327727316 3271 0
      0 AAEX03026041.1 dna:scaffold scaffold:CanFam3.1:AAEX03026041.1:1:3331:1 REF
      2327730587 3331 0
      0 AAEX03026040.1 dna:scaffold scaffold:CanFam3.1:AAEX03026040.1:1:3338:1 REF
      2327733918 3338 0
      0 AAEX03026039.1 dna:scaffold scaffold:CanFam3.1:AAEX03026039.1:1:3369:1 REF
      2327737256 3369 0
      0 AAEX03026038.1 dna:scaffold scaffold:CanFam3.1:AAEX03026038.1:1:3388:1 REF
      2327740625 3388 0
      0 AAEX03026037.1 dna:scaffold scaffold:CanFam3.1:AAEX03026037.1:1:3393:1 REF
      2327744013 3393 0
      0 AAEX03026036.1 dna:scaffold scaffold:CanFam3.1:AAEX03026036.1:1:3398:1 REF
      2327747406 3398 0
      0 AAEX03026035.1 dna:scaffold scaffold:CanFam3.1:AAEX03026035.1:1:3407:1 REF
      2327750804 3407 0
      0 AAEX03026034.1 dna:scaffold scaffold:CanFam3.1:AAEX03026034.1:1:3430:1 REF
      2327754211 3430 0
      0 AAEX03026033.1 dna:scaffold scaffold:CanFam3.1:AAEX03026033.1:1:3431:1 REF
      2327757641 3431 0
      0 AAEX03026032.1 dna:scaffold scaffold:CanFam3.1:AAEX03026032.1:1:3440:1 REF
      2327761072 3440 0
      0 AAEX03026031.1 dna:scaffold scaffold:CanFam3.1:AAEX03026031.1:1:3479:1 REF
      2327764512 3479 0
      0 AAEX03026030.1 dna:scaffold scaffold:CanFam3.1:AAEX03026030.1:1:3480:1 REF
      2327767991 3480 0
      0 AAEX03026029.1 dna:scaffold scaffold:CanFam3.1:AAEX03026029.1:1:3484:1 REF
      2327771471 3484 0
      0 AAEX03026028.1 dna:scaffold scaffold:CanFam3.1:AAEX03026028.1:1:3506:1 REF
      2327774955 3506 0
      0 AAEX03026027.1 dna:scaffold scaffold:CanFam3.1:AAEX03026027.1:1:3524:1 REF
      2327778461 3524 0
      0 AAEX03026026.1 dna:scaffold scaffold:CanFam3.1:AAEX03026026.1:1:3564:1 REF
      2327781985 3564 0
      0 AAEX03026025.1 dna:scaffold scaffold:CanFam3.1:AAEX03026025.1:1:3576:1 REF
      2327785549 3576 0
      0 AAEX03026024.1 dna:scaffold scaffold:CanFam3.1:AAEX03026024.1:1:3580:1 REF
      2327789125 3580 0
      0 AAEX03026023.1 dna:scaffold scaffold:CanFam3.1:AAEX03026023.1:1:3585:1 REF
      2327792705 3585 0
      0 AAEX03026022.1 dna:scaffold scaffold:CanFam3.1:AAEX03026022.1:1:3589:1 REF
      2327796290 3589 0
      0 AAEX03026021.1 dna:scaffold scaffold:CanFam3.1:AAEX03026021.1:1:3595:1 REF
      2327799879 3595 0
      0 AAEX03026020.1 dna:scaffold scaffold:CanFam3.1:AAEX03026020.1:1:3614:1 REF
      2327803474 3614 0
      0 AAEX03026019.1 dna:scaffold scaffold:CanFam3.1:AAEX03026019.1:1:3616:1 REF
      2327807088 3616 0
      0 AAEX03026018.1 dna:scaffold scaffold:CanFam3.1:AAEX03026018.1:1:3622:1 REF
      2327810704 3622 0
      0 AAEX03026017.1 dna:scaffold scaffold:CanFam3.1:AAEX03026017.1:1:3629:1 REF
      2327814326 3629 0
      0 AAEX03026016.1 dna:scaffold scaffold:CanFam3.1:AAEX03026016.1:1:3649:1 REF
      2327817955 3649 0
      0 AAEX03026015.1 dna:scaffold scaffold:CanFam3.1:AAEX03026015.1:1:3652:1 REF
      2327821604 3652 0
      0 AAEX03026014.1 dna:scaffold scaffold:CanFam3.1:AAEX03026014.1:1:3695:1 REF
      2327825256 3695 0
      0 AAEX03026013.1 dna:scaffold scaffold:CanFam3.1:AAEX03026013.1:1:3700:1 REF
      2327828951 3700 0
      0 AAEX03026012.1 dna:scaffold scaffold:CanFam3.1:AAEX03026012.1:1:3706:1 REF
      2327832651 3706 0
      0 AAEX03026011.1 dna:scaffold scaffold:CanFam3.1:AAEX03026011.1:1:3718:1 REF
      2327836357 3718 0
      0 AAEX03026010.1 dna:scaffold scaffold:CanFam3.1:AAEX03026010.1:1:3719:1 REF
      2327840075 3719 0
      0 AAEX03026009.1 dna:scaffold scaffold:CanFam3.1:AAEX03026009.1:1:3733:1 REF
      2327843794 3733 0
      0 AAEX03026008.1 dna:scaffold scaffold:CanFam3.1:AAEX03026008.1:1:3750:1 REF
      2327847527 3750 0
      0 AAEX03026007.1 dna:scaffold scaffold:CanFam3.1:AAEX03026007.1:1:3761:1 REF
      2327851277 3761 0
      0 AAEX03026006.1 dna:scaffold scaffold:CanFam3.1:AAEX03026006.1:1:3777:1 REF
      2327855038 3777 0
      0 AAEX03026004.1 dna:scaffold scaffold:CanFam3.1:AAEX03026004.1:1:3780:1 REF
      2327858815 3780 0
      0 AAEX03026005.1 dna:scaffold scaffold:CanFam3.1:AAEX03026005.1:1:3780:1 REF
      2327862595 3780 0
      0 AAEX03026003.1 dna:scaffold scaffold:CanFam3.1:AAEX03026003.1:1:3817:1 REF
      2327866375 3817 0
      0 AAEX03026002.1 dna:scaffold scaffold:CanFam3.1:AAEX03026002.1:1:3847:1 REF
      2327870192 3847 0
      0 AAEX03026001.1 dna:scaffold scaffold:CanFam3.1:AAEX03026001.1:1:3848:1 REF
      2327874039 3848 0
      0 AAEX03026000.1 dna:scaffold scaffold:CanFam3.1:AAEX03026000.1:1:3849:1 REF
      2327877887 3849 0
      0 AAEX03025999.1 dna:scaffold scaffold:CanFam3.1:AAEX03025999.1:1:3919:1 REF
      2327881736 3919 0
      0 AAEX03025998.1 dna:scaffold scaffold:CanFam3.1:AAEX03025998.1:1:3925:1 REF
      2327885655 3925 0
      0 AAEX03025997.1 dna:scaffold scaffold:CanFam3.1:AAEX03025997.1:1:3928:1 REF
      2327889580 3928 0
      0 AAEX03025996.1 dna:scaffold scaffold:CanFam3.1:AAEX03025996.1:1:3941:1 REF
      2327893508 3941 0
      0 AAEX03025995.1 dna:scaffold scaffold:CanFam3.1:AAEX03025995.1:1:3948:1 REF
      2327897449 3948 0
      0 AAEX03025994.1 dna:scaffold scaffold:CanFam3.1:AAEX03025994.1:1:3976:1 REF
      2327901397 3976 0
      0 AAEX03025993.1 dna:scaffold scaffold:CanFam3.1:AAEX03025993.1:1:4001:1 REF
      2327905373 4001 0
      0 AAEX03025992.1 dna:scaffold scaffold:CanFam3.1:AAEX03025992.1:1:4011:1 REF
      2327909374 4011 0
      0 AAEX03025990.1 dna:scaffold scaffold:CanFam3.1:AAEX03025990.1:1:4025:1 REF
      2327913385 4025 0
      0 AAEX03025991.1 dna:scaffold scaffold:CanFam3.1:AAEX03025991.1:1:4025:1 REF
      2327917410 4025 0
      0 AAEX03025989.1 dna:scaffold scaffold:CanFam3.1:AAEX03025989.1:1:4060:1 REF
      2327921435 4060 0
      0 AAEX03025988.1 dna:scaffold scaffold:CanFam3.1:AAEX03025988.1:1:4061:1 REF
      2327925495 4061 0
      0 AAEX03025987.1 dna:scaffold scaffold:CanFam3.1:AAEX03025987.1:1:4064:1 REF
      2327929556 4064 0
      0 AAEX03025986.1 dna:scaffold scaffold:CanFam3.1:AAEX03025986.1:1:4070:1 REF
      2327933620 4070 0
      0 AAEX03025985.1 dna:scaffold scaffold:CanFam3.1:AAEX03025985.1:1:4096:1 REF
      2327937690 4096 0
      0 AAEX03025984.1 dna:scaffold scaffold:CanFam3.1:AAEX03025984.1:1:4097:1 REF
      2327941786 4097 0
      0 AAEX03025983.1 dna:scaffold scaffold:CanFam3.1:AAEX03025983.1:1:4112:1 REF
      2327945883 4112 0
      0 AAEX03025982.1 dna:scaffold scaffold:CanFam3.1:AAEX03025982.1:1:4117:1 REF
      2327949995 4117 0
      0 AAEX03025981.1 dna:scaffold scaffold:CanFam3.1:AAEX03025981.1:1:4121:1 REF
      2327954112 4121 0
      0 AAEX03025980.1 dna:scaffold scaffold:CanFam3.1:AAEX03025980.1:1:4123:1 REF
      2327958233 4123 0
      0 AAEX03025979.1 dna:scaffold scaffold:CanFam3.1:AAEX03025979.1:1:4126:1 REF
      2327962356 4126 0
      0 AAEX03025977.1 dna:scaffold scaffold:CanFam3.1:AAEX03025977.1:1:4130:1 REF
      2327966482 4130 0
      0 AAEX03025978.1 dna:scaffold scaffold:CanFam3.1:AAEX03025978.1:1:4130:1 REF
      2327970612 4130 0
      0 AAEX03025976.1 dna:scaffold scaffold:CanFam3.1:AAEX03025976.1:1:4141:1 REF
      2327974742 4141 0
      0 AAEX03025975.1 dna:scaffold scaffold:CanFam3.1:AAEX03025975.1:1:4156:1 REF
      2327978883 4156 0
      0 AAEX03025974.1 dna:scaffold scaffold:CanFam3.1:AAEX03025974.1:1:4163:1 REF
      2327983039 4163 0
      0 AAEX03025973.1 dna:scaffold scaffold:CanFam3.1:AAEX03025973.1:1:4172:1 REF
      2327987202 4172 0
      0 AAEX03025972.1 dna:scaffold scaffold:CanFam3.1:AAEX03025972.1:1:4177:1 REF
      2327991374 4177 0
      0 AAEX03025971.1 dna:scaffold scaffold:CanFam3.1:AAEX03025971.1:1:4183:1 REF
      2327995551 4183 0
      0 AAEX03025970.1 dna:scaffold scaffold:CanFam3.1:AAEX03025970.1:1:4186:1 REF
      2327999734 4186 0
      0 AAEX03025969.1 dna:scaffold scaffold:CanFam3.1:AAEX03025969.1:1:4193:1 REF
      2328003920 4193 0
      0 AAEX03025968.1 dna:scaffold scaffold:CanFam3.1:AAEX03025968.1:1:4198:1 REF
      2328008113 4198 0
      0 AAEX03025967.1 dna:scaffold scaffold:CanFam3.1:AAEX03025967.1:1:4201:1 REF
      2328012311 4201 0
      0 AAEX03025966.1 dna:scaffold scaffold:CanFam3.1:AAEX03025966.1:1:4225:1 REF
      2328016512 4225 0
      0 AAEX03025965.1 dna:scaffold scaffold:CanFam3.1:AAEX03025965.1:1:4231:1 REF
      2328020737 4231 0
      0 AAEX03025964.1 dna:scaffold scaffold:CanFam3.1:AAEX03025964.1:1:4233:1 REF
      2328024968 4233 0
      0 AAEX03025963.1 dna:scaffold scaffold:CanFam3.1:AAEX03025963.1:1:4234:1 REF
      2328029201 4234 0
      0 AAEX03025962.1 dna:scaffold scaffold:CanFam3.1:AAEX03025962.1:1:4238:1 REF
      2328033435 4238 0
      0 AAEX03025961.1 dna:scaffold scaffold:CanFam3.1:AAEX03025961.1:1:4239:1 REF
      2328037673 4239 0
      0 AAEX03025960.1 dna:scaffold scaffold:CanFam3.1:AAEX03025960.1:1:4248:1 REF
      2328041912 4248 0
      0 AAEX03025959.1 dna:scaffold scaffold:CanFam3.1:AAEX03025959.1:1:4252:1 REF
      2328046160 4252 0
      0 AAEX03025957.1 dna:scaffold scaffold:CanFam3.1:AAEX03025957.1:1:4276:1 REF
      2328050412 4276 0
      0 AAEX03025958.1 dna:scaffold scaffold:CanFam3.1:AAEX03025958.1:1:4276:1 REF
      2328054688 4276 0
      0 AAEX03025956.1 dna:scaffold scaffold:CanFam3.1:AAEX03025956.1:1:4277:1 REF
      2328058964 4277 0
      0 AAEX03025955.1 dna:scaffold scaffold:CanFam3.1:AAEX03025955.1:1:4279:1 REF
      2328063241 4279 0
      0 AAEX03025954.1 dna:scaffold scaffold:CanFam3.1:AAEX03025954.1:1:4281:1 REF
      2328067520 4281 0
      0 AAEX03025953.1 dna:scaffold scaffold:CanFam3.1:AAEX03025953.1:1:4282:1 REF
      2328071801 4282 0
      0 AAEX03025952.1 dna:scaffold scaffold:CanFam3.1:AAEX03025952.1:1:4283:1 REF
      2328076083 4283 0
      0 AAEX03025951.1 dna:scaffold scaffold:CanFam3.1:AAEX03025951.1:1:4285:1 REF
      2328080366 4285 0
      0 AAEX03025950.1 dna:scaffold scaffold:CanFam3.1:AAEX03025950.1:1:4300:1 REF
      2328084651 4300 0
      0 AAEX03025949.1 dna:scaffold scaffold:CanFam3.1:AAEX03025949.1:1:4302:1 REF
      2328088951 4302 0
      0 AAEX03025948.1 dna:scaffold scaffold:CanFam3.1:AAEX03025948.1:1:4311:1 REF
      2328093253 4311 0
      0 AAEX03025947.1 dna:scaffold scaffold:CanFam3.1:AAEX03025947.1:1:4329:1 REF
      2328097564 4329 0
      0 AAEX03025946.1 dna:scaffold scaffold:CanFam3.1:AAEX03025946.1:1:4353:1 REF
      2328101893 4353 0
      0 AAEX03025945.1 dna:scaffold scaffold:CanFam3.1:AAEX03025945.1:1:4356:1 REF
      2328106246 4356 0
      0 AAEX03025944.1 dna:scaffold scaffold:CanFam3.1:AAEX03025944.1:1:4359:1 REF
      2328110602 4359 0
      0 AAEX03025943.1 dna:scaffold scaffold:CanFam3.1:AAEX03025943.1:1:4368:1 REF
      2328114961 4368 0
      0 AAEX03025942.1 dna:scaffold scaffold:CanFam3.1:AAEX03025942.1:1:4378:1 REF
      2328119329 4378 0
      0 AAEX03025941.1 dna:scaffold scaffold:CanFam3.1:AAEX03025941.1:1:4388:1 REF
      2328123707 4388 0
      0 AAEX03025939.1 dna:scaffold scaffold:CanFam3.1:AAEX03025939.1:1:4389:1 REF
      2328128095 4389 0
      0 AAEX03025940.1 dna:scaffold scaffold:CanFam3.1:AAEX03025940.1:1:4389:1 REF
      2328132484 4389 0
      0 AAEX03025938.1 dna:scaffold scaffold:CanFam3.1:AAEX03025938.1:1:4393:1 REF
      2328136873 4393 0
      0 AAEX03025937.1 dna:scaffold scaffold:CanFam3.1:AAEX03025937.1:1:4399:1 REF
      2328141266 4399 0
      0 AAEX03025936.1 dna:scaffold scaffold:CanFam3.1:AAEX03025936.1:1:4403:1 REF
      2328145665 4403 0
      0 AAEX03025934.1 dna:scaffold scaffold:CanFam3.1:AAEX03025934.1:1:4404:1 REF
      2328150068 4404 0
      0 AAEX03025935.1 dna:scaffold scaffold:CanFam3.1:AAEX03025935.1:1:4404:1 REF
      2328154472 4404 0
      0 AAEX03025933.1 dna:scaffold scaffold:CanFam3.1:AAEX03025933.1:1:4408:1 REF
      2328158876 4408 0
      0 AAEX03025931.1 dna:scaffold scaffold:CanFam3.1:AAEX03025931.1:1:4418:1 REF
      2328163284 4418 0
      0 AAEX03025932.1 dna:scaffold scaffold:CanFam3.1:AAEX03025932.1:1:4418:1 REF
      2328167702 4418 0
      0 AAEX03025930.1 dna:scaffold scaffold:CanFam3.1:AAEX03025930.1:1:4420:1 REF
      2328172120 4420 0
      0 AAEX03025929.1 dna:scaffold scaffold:CanFam3.1:AAEX03025929.1:1:4428:1 REF
      2328176540 4428 0
      0 AAEX03025928.1 dna:scaffold scaffold:CanFam3.1:AAEX03025928.1:1:4429:1 REF
      2328180968 4429 0
      0 AAEX03025927.1 dna:scaffold scaffold:CanFam3.1:AAEX03025927.1:1:4430:1 REF
      2328185397 4430 0
      0 AAEX03025926.1 dna:scaffold scaffold:CanFam3.1:AAEX03025926.1:1:4437:1 REF
      2328189827 4437 0
      0 AAEX03025925.1 dna:scaffold scaffold:CanFam3.1:AAEX03025925.1:1:4439:1 REF
      2328194264 4439 0
      0 AAEX03025924.1 dna:scaffold scaffold:CanFam3.1:AAEX03025924.1:1:4443:1 REF
      2328198703 4443 0
      0 AAEX03025923.1 dna:scaffold scaffold:CanFam3.1:AAEX03025923.1:1:4447:1 REF
      2328203146 4447 0
      0 AAEX03025922.1 dna:scaffold scaffold:CanFam3.1:AAEX03025922.1:1:4453:1 REF
      2328207593 4453 0
      0 AAEX03025921.1 dna:scaffold scaffold:CanFam3.1:AAEX03025921.1:1:4454:1 REF
      2328212046 4454 0
      0 AAEX03025920.1 dna:scaffold scaffold:CanFam3.1:AAEX03025920.1:1:4460:1 REF
      2328216500 4460 0
      0 AAEX03025919.1 dna:scaffold scaffold:CanFam3.1:AAEX03025919.1:1:4463:1 REF
      2328220960 4463 0
      0 AAEX03025918.1 dna:scaffold scaffold:CanFam3.1:AAEX03025918.1:1:4469:1 REF
      2328225423 4469 0
      0 AAEX03025917.1 dna:scaffold scaffold:CanFam3.1:AAEX03025917.1:1:4476:1 REF
      2328229892 4476 0
      0 AAEX03025916.1 dna:scaffold scaffold:CanFam3.1:AAEX03025916.1:1:4480:1 REF
      2328234368 4480 0
      0 AAEX03025915.1 dna:scaffold scaffold:CanFam3.1:AAEX03025915.1:1:4485:1 REF
      2328238848 4485 0
      0 AAEX03025914.1 dna:scaffold scaffold:CanFam3.1:AAEX03025914.1:1:4501:1 REF
      2328243333 4501 0
      0 AAEX03025913.1 dna:scaffold scaffold:CanFam3.1:AAEX03025913.1:1:4507:1 REF
      2328247834 4507 0
      0 AAEX03025912.1 dna:scaffold scaffold:CanFam3.1:AAEX03025912.1:1:4519:1 REF
      2328252341 4519 0
      0 AAEX03025911.1 dna:scaffold scaffold:CanFam3.1:AAEX03025911.1:1:4527:1 REF
      2328256860 4527 0
      0 AAEX03025910.1 dna:scaffold scaffold:CanFam3.1:AAEX03025910.1:1:4539:1 REF
      2328261387 4539 0
      0 AAEX03025909.1 dna:scaffold scaffold:CanFam3.1:AAEX03025909.1:1:4541:1 REF
      2328265926 4541 0
      0 AAEX03025908.1 dna:scaffold scaffold:CanFam3.1:AAEX03025908.1:1:4543:1 REF
      2328270467 4543 0
      0 AAEX03025907.1 dna:scaffold scaffold:CanFam3.1:AAEX03025907.1:1:4550:1 REF
      2328275010 4550 0
      0 AAEX03025906.1 dna:scaffold scaffold:CanFam3.1:AAEX03025906.1:1:4552:1 REF
      2328279560 4552 0
      0 AAEX03025905.1 dna:scaffold scaffold:CanFam3.1:AAEX03025905.1:1:4554:1 REF
      2328284112 4554 0
      0 AAEX03025904.1 dna:scaffold scaffold:CanFam3.1:AAEX03025904.1:1:4555:1 REF
      2328288666 4555 0
      0 AAEX03025903.1 dna:scaffold scaffold:CanFam3.1:AAEX03025903.1:1:4562:1 REF
      2328293221 4562 0
      0 AAEX03025901.1 dna:scaffold scaffold:CanFam3.1:AAEX03025901.1:1:4572:1 REF
      2328297783 4572 0
      0 AAEX03025902.1 dna:scaffold scaffold:CanFam3.1:AAEX03025902.1:1:4572:1 REF
      2328302355 4572 0
      0 AAEX03025900.1 dna:scaffold scaffold:CanFam3.1:AAEX03025900.1:1:4574:1 REF
      2328306927 4574 0
      0 AAEX03025899.1 dna:scaffold scaffold:CanFam3.1:AAEX03025899.1:1:4575:1 REF
      2328311501 4575 0
      0 AAEX03025896.1 dna:scaffold scaffold:CanFam3.1:AAEX03025896.1:1:4577:1 REF
      2328316076 4577 0
      0 AAEX03025897.1 dna:scaffold scaffold:CanFam3.1:AAEX03025897.1:1:4577:1 REF
      2328320653 4577 0
      0 AAEX03025898.1 dna:scaffold scaffold:CanFam3.1:AAEX03025898.1:1:4577:1 REF
      2328325230 4577 0
      0 AAEX03025895.1 dna:scaffold scaffold:CanFam3.1:AAEX03025895.1:1:4580:1 REF
      2328329807 4580 0
      0 AAEX03025894.1 dna:scaffold scaffold:CanFam3.1:AAEX03025894.1:1:4588:1 REF
      2328334387 4588 0
      0 AAEX03025893.1 dna:scaffold scaffold:CanFam3.1:AAEX03025893.1:1:4594:1 REF
      2328338975 4594 0
      0 AAEX03025891.1 dna:scaffold scaffold:CanFam3.1:AAEX03025891.1:1:4600:1 REF
      2328343569 4600 0
      0 AAEX03025892.1 dna:scaffold scaffold:CanFam3.1:AAEX03025892.1:1:4600:1 REF
      2328348169 4600 0
      0 AAEX03025890.1 dna:scaffold scaffold:CanFam3.1:AAEX03025890.1:1:4601:1 REF
      2328352769 4601 0
      0 AAEX03025889.1 dna:scaffold scaffold:CanFam3.1:AAEX03025889.1:1:4612:1 REF
      2328357370 4612 0
      0 AAEX03025888.1 dna:scaffold scaffold:CanFam3.1:AAEX03025888.1:1:4613:1 REF
      2328361982 4613 0
      0 AAEX03025887.1 dna:scaffold scaffold:CanFam3.1:AAEX03025887.1:1:4619:1 REF
      2328366595 4619 0
      0 AAEX03025886.1 dna:scaffold scaffold:CanFam3.1:AAEX03025886.1:1:4629:1 REF
      2328371214 4629 0
      0 AAEX03025885.1 dna:scaffold scaffold:CanFam3.1:AAEX03025885.1:1:4630:1 REF
      2328375843 4630 0
      0 AAEX03025884.1 dna:scaffold scaffold:CanFam3.1:AAEX03025884.1:1:4631:1 REF
      2328380473 4631 0
      0 AAEX03025883.1 dna:scaffold scaffold:CanFam3.1:AAEX03025883.1:1:4636:1 REF
      2328385104 4636 0
      0 AAEX03025882.1 dna:scaffold scaffold:CanFam3.1:AAEX03025882.1:1:4639:1 REF
      2328389740 4639 0
      0 AAEX03025880.1 dna:scaffold scaffold:CanFam3.1:AAEX03025880.1:1:4649:1 REF
      2328394379 4649 0
      0 AAEX03025881.1 dna:scaffold scaffold:CanFam3.1:AAEX03025881.1:1:4649:1 REF
      2328399028 4649 0
      0 AAEX03025879.1 dna:scaffold scaffold:CanFam3.1:AAEX03025879.1:1:4659:1 REF
      2328403677 4659 0
      0 AAEX03025878.1 dna:scaffold scaffold:CanFam3.1:AAEX03025878.1:1:4662:1 REF
      2328408336 4662 0
      0 AAEX03025877.1 dna:scaffold scaffold:CanFam3.1:AAEX03025877.1:1:4668:1 REF
      2328412998 4668 0
      0 AAEX03025876.1 dna:scaffold scaffold:CanFam3.1:AAEX03025876.1:1:4669:1 REF
      2328417666 4669 0
      0 AAEX03025874.1 dna:scaffold scaffold:CanFam3.1:AAEX03025874.1:1:4673:1 REF
      2328422335 4673 0
      0 AAEX03025875.1 dna:scaffold scaffold:CanFam3.1:AAEX03025875.1:1:4673:1 REF
      2328427008 4673 0
      0 AAEX03025873.1 dna:scaffold scaffold:CanFam3.1:AAEX03025873.1:1:4676:1 REF
      2328431681 4676 0
      0 AAEX03025872.1 dna:scaffold scaffold:CanFam3.1:AAEX03025872.1:1:4681:1 REF
      2328436357 4681 0
      0 AAEX03025871.1 dna:scaffold scaffold:CanFam3.1:AAEX03025871.1:1:4683:1 REF
      2328441038 4683 0
      0 AAEX03025870.1 dna:scaffold scaffold:CanFam3.1:AAEX03025870.1:1:4685:1 REF
      2328445721 4685 0
      0 AAEX03025869.1 dna:scaffold scaffold:CanFam3.1:AAEX03025869.1:1:4687:1 REF
      2328450406 4687 0
      0 AAEX03025868.1 dna:scaffold scaffold:CanFam3.1:AAEX03025868.1:1:4688:1 REF
      2328455093 4688 0
      0 AAEX03025867.1 dna:scaffold scaffold:CanFam3.1:AAEX03025867.1:1:4689:1 REF
      2328459781 4689 0
      0 AAEX03025866.1 dna:scaffold scaffold:CanFam3.1:AAEX03025866.1:1:4694:1 REF
      2328464470 4694 0
      0 AAEX03025865.1 dna:scaffold scaffold:CanFam3.1:AAEX03025865.1:1:4695:1 REF
      2328469164 4695 0
      0 AAEX03025864.1 dna:scaffold scaffold:CanFam3.1:AAEX03025864.1:1:4698:1 REF
      2328473859 4698 0
      0 AAEX03025862.1 dna:scaffold scaffold:CanFam3.1:AAEX03025862.1:1:4702:1 REF
      2328478557 4702 0
      0 AAEX03025863.1 dna:scaffold scaffold:CanFam3.1:AAEX03025863.1:1:4702:1 REF
      2328483259 4702 0
      0 AAEX03025861.1 dna:scaffold scaffold:CanFam3.1:AAEX03025861.1:1:4717:1 REF
      2328487961 4717 0
      0 AAEX03025860.1 dna:scaffold scaffold:CanFam3.1:AAEX03025860.1:1:4718:1 REF
      2328492678 4718 0
      0 AAEX03025859.1 dna:scaffold scaffold:CanFam3.1:AAEX03025859.1:1:4725:1 REF
      2328497396 4725 0
      0 AAEX03025858.1 dna:scaffold scaffold:CanFam3.1:AAEX03025858.1:1:4726:1 REF
      2328502121 4726 0
      0 AAEX03025857.1 dna:scaffold scaffold:CanFam3.1:AAEX03025857.1:1:4732:1 REF
      2328506847 4732 0
      0 AAEX03025856.1 dna:scaffold scaffold:CanFam3.1:AAEX03025856.1:1:4733:1 REF
      2328511579 4733 0
      0 AAEX03025855.1 dna:scaffold scaffold:CanFam3.1:AAEX03025855.1:1:4734:1 REF
      2328516312 4734 0
      0 AAEX03025852.1 dna:scaffold scaffold:CanFam3.1:AAEX03025852.1:1:4736:1 REF
      2328521046 4736 0
      0 AAEX03025853.1 dna:scaffold scaffold:CanFam3.1:AAEX03025853.1:1:4736:1 REF
      2328525782 4736 0
      0 AAEX03025854.1 dna:scaffold scaffold:CanFam3.1:AAEX03025854.1:1:4736:1 REF
      2328530518 4736 0
      0 AAEX03025851.1 dna:scaffold scaffold:CanFam3.1:AAEX03025851.1:1:4737:1 REF
      2328535254 4737 0
      0 AAEX03025850.1 dna:scaffold scaffold:CanFam3.1:AAEX03025850.1:1:4738:1 REF
      2328539991 4738 0
      0 AAEX03025849.1 dna:scaffold scaffold:CanFam3.1:AAEX03025849.1:1:4743:1 REF
      2328544729 4743 0
      0 AAEX03025848.1 dna:scaffold scaffold:CanFam3.1:AAEX03025848.1:1:4744:1 REF
      2328549472 4744 0
      0 AAEX03025847.1 dna:scaffold scaffold:CanFam3.1:AAEX03025847.1:1:4758:1 REF
      2328554216 4758 0
      0 AAEX03025846.1 dna:scaffold scaffold:CanFam3.1:AAEX03025846.1:1:4767:1 REF
      2328558974 4767 0
      0 AAEX03025845.1 dna:scaffold scaffold:CanFam3.1:AAEX03025845.1:1:4781:1 REF
      2328563741 4781 0
      0 AAEX03025844.1 dna:scaffold scaffold:CanFam3.1:AAEX03025844.1:1:4782:1 REF
      2328568522 4782 0
      0 AAEX03025843.1 dna:scaffold scaffold:CanFam3.1:AAEX03025843.1:1:4783:1 REF
      2328573304 4783 0
      0 AAEX03025841.1 dna:scaffold scaffold:CanFam3.1:AAEX03025841.1:1:4784:1 REF
      2328578087 4784 0
      0 AAEX03025842.1 dna:scaffold scaffold:CanFam3.1:AAEX03025842.1:1:4784:1 REF
      2328582871 4784 0
      0 AAEX03025840.1 dna:scaffold scaffold:CanFam3.1:AAEX03025840.1:1:4787:1 REF
      2328587655 4787 0
      0 AAEX03025839.1 dna:scaffold scaffold:CanFam3.1:AAEX03025839.1:1:4789:1 REF
      2328592442 4789 0
      0 AAEX03025837.1 dna:scaffold scaffold:CanFam3.1:AAEX03025837.1:1:4792:1 REF
      2328597231 4792 0
      0 AAEX03025838.1 dna:scaffold scaffold:CanFam3.1:AAEX03025838.1:1:4792:1 REF
      2328602023 4792 0
      0 AAEX03025835.1 dna:scaffold scaffold:CanFam3.1:AAEX03025835.1:1:4798:1 REF
      2328606815 4798 0
      0 AAEX03025836.1 dna:scaffold scaffold:CanFam3.1:AAEX03025836.1:1:4798:1 REF
      2328611613 4798 0
      0 AAEX03025834.1 dna:scaffold scaffold:CanFam3.1:AAEX03025834.1:1:4809:1 REF
      2328616411 4809 0
      0 AAEX03025833.1 dna:scaffold scaffold:CanFam3.1:AAEX03025833.1:1:4818:1 REF
      2328621220 4818 0
      0 AAEX03025832.1 dna:scaffold scaffold:CanFam3.1:AAEX03025832.1:1:4828:1 REF
      2328626038 4828 0
      0 AAEX03025831.1 dna:scaffold scaffold:CanFam3.1:AAEX03025831.1:1:4835:1 REF
      2328630866 4835 0
      0 AAEX03025830.1 dna:scaffold scaffold:CanFam3.1:AAEX03025830.1:1:4838:1 REF
      2328635701 4838 0
      0 AAEX03025829.1 dna:scaffold scaffold:CanFam3.1:AAEX03025829.1:1:4840:1 REF
      2328640539 4840 0
      0 AAEX03025828.1 dna:scaffold scaffold:CanFam3.1:AAEX03025828.1:1:4847:1 REF
      2328645379 4847 0
      0 AAEX03025827.1 dna:scaffold scaffold:CanFam3.1:AAEX03025827.1:1:4853:1 REF
      2328650226 4853 0
      0 AAEX03025826.1 dna:scaffold scaffold:CanFam3.1:AAEX03025826.1:1:4854:1 REF
      2328655079 4854 0
      0 AAEX03025825.1 dna:scaffold scaffold:CanFam3.1:AAEX03025825.1:1:4856:1 REF
      2328659933 4856 0
      0 AAEX03025824.1 dna:scaffold scaffold:CanFam3.1:AAEX03025824.1:1:4862:1 REF
      2328664789 4862 0
      0 AAEX03025823.1 dna:scaffold scaffold:CanFam3.1:AAEX03025823.1:1:4863:1 REF
      2328669651 4863 0
      0 AAEX03025822.1 dna:scaffold scaffold:CanFam3.1:AAEX03025822.1:1:4867:1 REF
      2328674514 4867 0
      0 AAEX03025821.1 dna:scaffold scaffold:CanFam3.1:AAEX03025821.1:1:4868:1 REF
      2328679381 4868 0
      0 AAEX03025820.1 dna:scaffold scaffold:CanFam3.1:AAEX03025820.1:1:4869:1 REF
      2328684249 4869 0
      0 AAEX03025819.1 dna:scaffold scaffold:CanFam3.1:AAEX03025819.1:1:4870:1 REF
      2328689118 4870 0
      0 AAEX03025818.1 dna:scaffold scaffold:CanFam3.1:AAEX03025818.1:1:4871:1 REF
      2328693988 4871 0
      0 AAEX03025817.1 dna:scaffold scaffold:CanFam3.1:AAEX03025817.1:1:4874:1 REF
      2328698859 4874 0
      0 AAEX03025816.1 dna:scaffold scaffold:CanFam3.1:AAEX03025816.1:1:4877:1 REF
      2328703733 4877 0
      0 AAEX03025815.1 dna:scaffold scaffold:CanFam3.1:AAEX03025815.1:1:4879:1 REF
      2328708610 4879 0
      0 AAEX03025814.1 dna:scaffold scaffold:CanFam3.1:AAEX03025814.1:1:4889:1 REF
      2328713489 4889 0
      0 AAEX03025812.1 dna:scaffold scaffold:CanFam3.1:AAEX03025812.1:1:4892:1 REF
      2328718378 4892 0
      0 AAEX03025813.1 dna:scaffold scaffold:CanFam3.1:AAEX03025813.1:1:4892:1 REF
      2328723270 4892 0
      0 AAEX03025811.1 dna:scaffold scaffold:CanFam3.1:AAEX03025811.1:1:4893:1 REF
      2328728162 4893 0
      0 AAEX03025810.1 dna:scaffold scaffold:CanFam3.1:AAEX03025810.1:1:4894:1 REF
      2328733055 4894 0
      0 AAEX03025808.1 dna:scaffold scaffold:CanFam3.1:AAEX03025808.1:1:4911:1 REF
      2328737949 4911 0
      0 AAEX03025809.1 dna:scaffold scaffold:CanFam3.1:AAEX03025809.1:1:4911:1 REF
      2328742860 4911 0
      0 AAEX03025807.1 dna:scaffold scaffold:CanFam3.1:AAEX03025807.1:1:4914:1 REF
      2328747771 4914 0
      0 AAEX03025806.1 dna:scaffold scaffold:CanFam3.1:AAEX03025806.1:1:4921:1 REF
      2328752685 4921 0
      0 AAEX03025805.1 dna:scaffold scaffold:CanFam3.1:AAEX03025805.1:1:4922:1 REF
      2328757606 4922 0
      0 AAEX03025804.1 dna:scaffold scaffold:CanFam3.1:AAEX03025804.1:1:4925:1 REF
      2328762528 4925 0
      0 AAEX03025803.1 dna:scaffold scaffold:CanFam3.1:AAEX03025803.1:1:4929:1 REF
      2328767453 4929 0
      0 AAEX03025802.1 dna:scaffold scaffold:CanFam3.1:AAEX03025802.1:1:4934:1 REF
      2328772382 4934 0
      0 AAEX03025801.1 dna:scaffold scaffold:CanFam3.1:AAEX03025801.1:1:4941:1 REF
      2328777316 4941 0
      0 AAEX03025800.1 dna:scaffold scaffold:CanFam3.1:AAEX03025800.1:1:4950:1 REF
      2328782257 4950 0
      0 AAEX03025799.1 dna:scaffold scaffold:CanFam3.1:AAEX03025799.1:1:4952:1 REF
      2328787207 4952 0
      0 AAEX03025798.1 dna:scaffold scaffold:CanFam3.1:AAEX03025798.1:1:4955:1 REF
      2328792159 4955 0
      0 AAEX03025796.1 dna:scaffold scaffold:CanFam3.1:AAEX03025796.1:1:4956:1 REF
      2328797114 4956 0
      0 AAEX03025797.1 dna:scaffold scaffold:CanFam3.1:AAEX03025797.1:1:4956:1 REF
      2328802070 4956 0
      0 AAEX03025792.1 dna:scaffold scaffold:CanFam3.1:AAEX03025792.1:1:4957:1 REF
      2328807026 4957 0
      0 AAEX03025795.1 dna:scaffold scaffold:CanFam3.1:AAEX03025795.1:1:4957:1 REF
      2328811983 4957 0
      0 AAEX03025791.1 dna:scaffold scaffold:CanFam3.1:AAEX03025791.1:1:4959:1 REF
      2328816940 4959 0
      0 AAEX03025789.1 dna:scaffold scaffold:CanFam3.1:AAEX03025789.1:1:4960:1 REF
      2328821899 4960 0
      0 AAEX03025790.1 dna:scaffold scaffold:CanFam3.1:AAEX03025790.1:1:4960:1 REF
      2328826859 4960 0
      0 AAEX03025788.1 dna:scaffold scaffold:CanFam3.1:AAEX03025788.1:1:4965:1 REF
      2328831819 4965 0
      0 AAEX03025787.1 dna:scaffold scaffold:CanFam3.1:AAEX03025787.1:1:4967:1 REF
      2328836784 4967 0
      0 AAEX03025784.1 dna:scaffold scaffold:CanFam3.1:AAEX03025784.1:1:4969:1 REF
      2328841751 4969 0
      0 AAEX03025785.1 dna:scaffold scaffold:CanFam3.1:AAEX03025785.1:1:4969:1 REF
      2328846720 4969 0
      0 AAEX03025786.1 dna:scaffold scaffold:CanFam3.1:AAEX03025786.1:1:4969:1 REF
      2328851689 4969 0
      0 AAEX03025783.1 dna:scaffold scaffold:CanFam3.1:AAEX03025783.1:1:4974:1 REF
      2328856658 4974 0
      0 AAEX03025781.1 dna:scaffold scaffold:CanFam3.1:AAEX03025781.1:1:4975:1 REF
      2328861632 4975 0
      0 AAEX03025782.1 dna:scaffold scaffold:CanFam3.1:AAEX03025782.1:1:4975:1 REF
      2328866607 4975 0
      0 AAEX03025780.1 dna:scaffold scaffold:CanFam3.1:AAEX03025780.1:1:4980:1 REF
      2328871582 4980 0
      0 AAEX03025779.1 dna:scaffold scaffold:CanFam3.1:AAEX03025779.1:1:4984:1 REF
      2328876562 4984 0
      0 AAEX03025778.1 dna:scaffold scaffold:CanFam3.1:AAEX03025778.1:1:4988:1 REF
      2328881546 4988 0
      0 AAEX03025777.1 dna:scaffold scaffold:CanFam3.1:AAEX03025777.1:1:4989:1 REF
      2328886534 4989 0
      0 AAEX03025776.1 dna:scaffold scaffold:CanFam3.1:AAEX03025776.1:1:4991:1 REF
      2328891523 4991 0
      0 AAEX03025775.1 dna:scaffold scaffold:CanFam3.1:AAEX03025775.1:1:4993:1 REF
      2328896514 4993 0
      0 AAEX03025774.1 dna:scaffold scaffold:CanFam3.1:AAEX03025774.1:1:4994:1 REF
      2328901507 4994 0
      0 AAEX03025773.1 dna:scaffold scaffold:CanFam3.1:AAEX03025773.1:1:4995:1 REF
      2328906501 4995 0
      0 AAEX03025772.1 dna:scaffold scaffold:CanFam3.1:AAEX03025772.1:1:4998:1 REF
      2328911496 4998 0
      0 AAEX03025771.1 dna:scaffold scaffold:CanFam3.1:AAEX03025771.1:1:5000:1 REF
      2328916494 5000 0
      0 AAEX03025770.1 dna:scaffold scaffold:CanFam3.1:AAEX03025770.1:1:5004:1 REF
      2328921494 5004 0
      0 AAEX03025769.1 dna:scaffold scaffold:CanFam3.1:AAEX03025769.1:1:5007:1 REF
      2328926498 5007 0
      0 AAEX03025768.1 dna:scaffold scaffold:CanFam3.1:AAEX03025768.1:1:5010:1 REF
      2328931505 5010 0
      0 AAEX03025767.1 dna:scaffold scaffold:CanFam3.1:AAEX03025767.1:1:5015:1 REF
      2328936515 5015 0
      0 AAEX03025766.1 dna:scaffold scaffold:CanFam3.1:AAEX03025766.1:1:5016:1 REF
      2328941530 5016 0
      0 AAEX03025765.1 dna:scaffold scaffold:CanFam3.1:AAEX03025765.1:1:5017:1 REF
      2328946546 5017 0
      0 AAEX03025764.1 dna:scaffold scaffold:CanFam3.1:AAEX03025764.1:1:5018:1 REF
      2328951563 5018 0
      0 AAEX03025763.1 dna:scaffold scaffold:CanFam3.1:AAEX03025763.1:1:5020:1 REF
      2328956581 5020 0
      0 AAEX03025762.1 dna:scaffold scaffold:CanFam3.1:AAEX03025762.1:1:5021:1 REF
      2328961601 5021 0
      0 AAEX03025761.1 dna:scaffold scaffold:CanFam3.1:AAEX03025761.1:1:5031:1 REF
      2328966622 5031 0
      0 AAEX03025760.1 dna:scaffold scaffold:CanFam3.1:AAEX03025760.1:1:5038:1 REF
      2328971653 5038 0
      0 AAEX03025759.1 dna:scaffold scaffold:CanFam3.1:AAEX03025759.1:1:5041:1 REF
      2328976691 5041 0
      0 AAEX03025758.1 dna:scaffold scaffold:CanFam3.1:AAEX03025758.1:1:5046:1 REF
      2328981732 5046 0
      0 AAEX03025757.1 dna:scaffold scaffold:CanFam3.1:AAEX03025757.1:1:5051:1 REF
      2328986778 5051 0
      0 AAEX03025756.1 dna:scaffold scaffold:CanFam3.1:AAEX03025756.1:1:5053:1 REF
      2328991829 5053 0
      0 AAEX03025754.1 dna:scaffold scaffold:CanFam3.1:AAEX03025754.1:1:5054:1 REF
      2328996882 5054 0
      0 AAEX03025755.1 dna:scaffold scaffold:CanFam3.1:AAEX03025755.1:1:5054:1 REF
      2329001936 5054 0
      0 AAEX03025753.1 dna:scaffold scaffold:CanFam3.1:AAEX03025753.1:1:5059:1 REF
      2329006990 5059 0
      0 AAEX03025752.1 dna:scaffold scaffold:CanFam3.1:AAEX03025752.1:1:5061:1 REF
      2329012049 5061 0
      0 AAEX03025751.1 dna:scaffold scaffold:CanFam3.1:AAEX03025751.1:1:5068:1 REF
      2329017110 5068 0
      0 AAEX03025750.1 dna:scaffold scaffold:CanFam3.1:AAEX03025750.1:1:5070:1 REF
      2329022178 5070 0
      0 AAEX03025749.1 dna:scaffold scaffold:CanFam3.1:AAEX03025749.1:1:5073:1 REF
      2329027248 5073 0
      0 AAEX03025748.1 dna:scaffold scaffold:CanFam3.1:AAEX03025748.1:1:5080:1 REF
      2329032321 5080 0
      0 AAEX03025747.1 dna:scaffold scaffold:CanFam3.1:AAEX03025747.1:1:5089:1 REF
      2329037401 5089 0
      0 AAEX03025746.1 dna:scaffold scaffold:CanFam3.1:AAEX03025746.1:1:5096:1 REF
      2329042490 5096 0
      0 AAEX03025745.1 dna:scaffold scaffold:CanFam3.1:AAEX03025745.1:1:5099:1 REF
      2329047586 5099 0
      0 AAEX03025744.1 dna:scaffold scaffold:CanFam3.1:AAEX03025744.1:1:5110:1 REF
      2329052685 5110 0
      0 AAEX03025743.1 dna:scaffold scaffold:CanFam3.1:AAEX03025743.1:1:5112:1 REF
      2329057795 5112 0
      0 AAEX03025741.1 dna:scaffold scaffold:CanFam3.1:AAEX03025741.1:1:5115:1 REF
      2329062907 5115 0
      0 AAEX03025742.1 dna:scaffold scaffold:CanFam3.1:AAEX03025742.1:1:5115:1 REF
      2329068022 5115 0
      0 AAEX03025739.1 dna:scaffold scaffold:CanFam3.1:AAEX03025739.1:1:5118:1 REF
      2329073137 5118 0
      0 AAEX03025740.1 dna:scaffold scaffold:CanFam3.1:AAEX03025740.1:1:5118:1 REF
      2329078255 5118 0
      0 AAEX03025738.1 dna:scaffold scaffold:CanFam3.1:AAEX03025738.1:1:5119:1 REF
      2329083373 5119 0
      0 AAEX03025737.1 dna:scaffold scaffold:CanFam3.1:AAEX03025737.1:1:5123:1 REF
      2329088492 5123 0
      0 AAEX03025736.1 dna:scaffold scaffold:CanFam3.1:AAEX03025736.1:1:5124:1 REF
      2329093615 5124 0
      0 AAEX03025735.1 dna:scaffold scaffold:CanFam3.1:AAEX03025735.1:1:5129:1 REF
      2329098739 5129 0
      0 AAEX03025734.1 dna:scaffold scaffold:CanFam3.1:AAEX03025734.1:1:5130:1 REF
      2329103868 5130 0
      0 AAEX03025733.1 dna:scaffold scaffold:CanFam3.1:AAEX03025733.1:1:5132:1 REF
      2329108998 5132 0
      0 AAEX03025732.1 dna:scaffold scaffold:CanFam3.1:AAEX03025732.1:1:5136:1 REF
      2329114130 5136 0
      0 AAEX03025731.1 dna:scaffold scaffold:CanFam3.1:AAEX03025731.1:1:5137:1 REF
      2329119266 5137 0
      0 AAEX03025729.1 dna:scaffold scaffold:CanFam3.1:AAEX03025729.1:1:5147:1 REF
      2329124403 5147 0
      0 AAEX03025730.1 dna:scaffold scaffold:CanFam3.1:AAEX03025730.1:1:5147:1 REF
      2329129550 5147 0
      0 AAEX03025728.1 dna:scaffold scaffold:CanFam3.1:AAEX03025728.1:1:5150:1 REF
      2329134697 5150 0
      0 AAEX03025727.1 dna:scaffold scaffold:CanFam3.1:AAEX03025727.1:1:5153:1 REF
      2329139847 5153 0
      0 AAEX03025725.1 dna:scaffold scaffold:CanFam3.1:AAEX03025725.1:1:5154:1 REF
      2329145000 5154 0
      0 AAEX03025726.1 dna:scaffold scaffold:CanFam3.1:AAEX03025726.1:1:5154:1 REF
      2329150154 5154 0
      0 AAEX03025724.1 dna:scaffold scaffold:CanFam3.1:AAEX03025724.1:1:5167:1 REF
      2329155308 5167 0
      0 AAEX03025723.1 dna:scaffold scaffold:CanFam3.1:AAEX03025723.1:1:5169:1 REF
      2329160475 5169 0
      0 AAEX03025722.1 dna:scaffold scaffold:CanFam3.1:AAEX03025722.1:1:5173:1 REF
      2329165644 5173 0
      0 AAEX03025720.1 dna:scaffold scaffold:CanFam3.1:AAEX03025720.1:1:5174:1 REF
      2329170817 5174 0
      0 AAEX03025721.1 dna:scaffold scaffold:CanFam3.1:AAEX03025721.1:1:5174:1 REF
      2329175991 5174 0
      0 AAEX03025719.1 dna:scaffold scaffold:CanFam3.1:AAEX03025719.1:1:5180:1 REF
      2329181165 5180 0
      0 AAEX03025718.1 dna:scaffold scaffold:CanFam3.1:AAEX03025718.1:1:5185:1 REF
      2329186345 5185 0
      0 AAEX03025717.1 dna:scaffold scaffold:CanFam3.1:AAEX03025717.1:1:5186:1 REF
      2329191530 5186 0
      0 AAEX03025715.1 dna:scaffold scaffold:CanFam3.1:AAEX03025715.1:1:5188:1 REF
      2329196716 5188 0
      0 AAEX03025716.1 dna:scaffold scaffold:CanFam3.1:AAEX03025716.1:1:5188:1 REF
      2329201904 5188 0
      0 AAEX03025714.1 dna:scaffold scaffold:CanFam3.1:AAEX03025714.1:1:5193:1 REF
      2329207092 5193 0
      0 AAEX03025713.1 dna:scaffold scaffold:CanFam3.1:AAEX03025713.1:1:5208:1 REF
      2329212285 5208 0
      0 AAEX03025712.1 dna:scaffold scaffold:CanFam3.1:AAEX03025712.1:1:5212:1 REF
      2329217493 5212 0
      0 AAEX03025709.1 dna:scaffold scaffold:CanFam3.1:AAEX03025709.1:1:5222:1 REF
      2329222705 5222 0
      0 AAEX03025708.1 dna:scaffold scaffold:CanFam3.1:AAEX03025708.1:1:5224:1 REF
      2329227927 5224 0
      0 AAEX03025707.1 dna:scaffold scaffold:CanFam3.1:AAEX03025707.1:1:5235:1 REF
      2329233151 5235 0
      0 AAEX03025706.1 dna:scaffold scaffold:CanFam3.1:AAEX03025706.1:1:5240:1 REF
      2329238386 5240 0
      0 AAEX03025705.1 dna:scaffold scaffold:CanFam3.1:AAEX03025705.1:1:5241:1 REF
      2329243626 5241 0
      0 AAEX03025704.1 dna:scaffold scaffold:CanFam3.1:AAEX03025704.1:1:5243:1 REF
      2329248867 5243 0
      0 AAEX03025703.1 dna:scaffold scaffold:CanFam3.1:AAEX03025703.1:1:5244:1 REF
      2329254110 5244 0
      0 AAEX03025702.1 dna:scaffold scaffold:CanFam3.1:AAEX03025702.1:1:5246:1 REF
      2329259354 5246 0
      0 AAEX03025701.1 dna:scaffold scaffold:CanFam3.1:AAEX03025701.1:1:5253:1 REF
      2329264600 5253 0
      0 AAEX03025700.1 dna:scaffold scaffold:CanFam3.1:AAEX03025700.1:1:5254:1 REF
      2329269853 5254 0
      0 AAEX03025699.1 dna:scaffold scaffold:CanFam3.1:AAEX03025699.1:1:5257:1 REF
      2329275107 5257 0
      0 AAEX03025698.1 dna:scaffold scaffold:CanFam3.1:AAEX03025698.1:1:5258:1 REF
      2329280364 5258 0
      0 AAEX03025696.1 dna:scaffold scaffold:CanFam3.1:AAEX03025696.1:1:5261:1 REF
      2329285622 5261 0
      0 AAEX03025697.1 dna:scaffold scaffold:CanFam3.1:AAEX03025697.1:1:5261:1 REF
      2329290883 5261 0
      0 AAEX03025695.1 dna:scaffold scaffold:CanFam3.1:AAEX03025695.1:1:5262:1 REF
      2329296144 5262 0
      0 AAEX03025694.1 dna:scaffold scaffold:CanFam3.1:AAEX03025694.1:1:5264:1 REF
      2329301406 5264 0
      0 AAEX03025693.1 dna:scaffold scaffold:CanFam3.1:AAEX03025693.1:1:5272:1 REF
      2329306670 5272 0
      0 AAEX03025692.1 dna:scaffold scaffold:CanFam3.1:AAEX03025692.1:1:5276:1 REF
      2329311942 5276 0
      0 AAEX03025691.1 dna:scaffold scaffold:CanFam3.1:AAEX03025691.1:1:5277:1 REF
      2329317218 5277 0
      0 AAEX03025690.1 dna:scaffold scaffold:CanFam3.1:AAEX03025690.1:1:5283:1 REF
      2329322495 5283 0
      0 AAEX03025689.1 dna:scaffold scaffold:CanFam3.1:AAEX03025689.1:1:5288:1 REF
      2329327778 5288 0
      0 AAEX03025687.1 dna:scaffold scaffold:CanFam3.1:AAEX03025687.1:1:5289:1 REF
      2329333066 5289 0
      0 AAEX03025688.1 dna:scaffold scaffold:CanFam3.1:AAEX03025688.1:1:5289:1 REF
      2329338355 5289 0
      0 AAEX03025686.1 dna:scaffold scaffold:CanFam3.1:AAEX03025686.1:1:5290:1 REF
      2329343644 5290 0
      0 AAEX03025685.1 dna:scaffold scaffold:CanFam3.1:AAEX03025685.1:1:5291:1 REF
      2329348934 5291 0
      0 AAEX03025682.1 dna:scaffold scaffold:CanFam3.1:AAEX03025682.1:1:5292:1 REF
      2329354225 5292 0
      0 AAEX03025683.1 dna:scaffold scaffold:CanFam3.1:AAEX03025683.1:1:5292:1 REF
      2329359517 5292 0
      0 AAEX03025684.1 dna:scaffold scaffold:CanFam3.1:AAEX03025684.1:1:5292:1 REF
      2329364809 5292 0
      0 AAEX03025680.1 dna:scaffold scaffold:CanFam3.1:AAEX03025680.1:1:5295:1 REF
      2329370101 5295 0
      0 AAEX03025681.1 dna:scaffold scaffold:CanFam3.1:AAEX03025681.1:1:5295:1 REF
      2329375396 5295 0
      0 AAEX03025679.1 dna:scaffold scaffold:CanFam3.1:AAEX03025679.1:1:5298:1 REF
      2329380691 5298 0
      0 AAEX03025678.1 dna:scaffold scaffold:CanFam3.1:AAEX03025678.1:1:5302:1 REF
      2329385989 5302 0
      0 AAEX03025677.1 dna:scaffold scaffold:CanFam3.1:AAEX03025677.1:1:5303:1 REF
      2329391291 5303 0
      0 AAEX03025676.1 dna:scaffold scaffold:CanFam3.1:AAEX03025676.1:1:5307:1 REF
      2329396594 5307 0
      0 AAEX03025675.1 dna:scaffold scaffold:CanFam3.1:AAEX03025675.1:1:5317:1 REF
      2329401901 5317 0
      0 AAEX03025672.1 dna:scaffold scaffold:CanFam3.1:AAEX03025672.1:1:5321:1 REF
      2329407218 5321 0
      0 AAEX03025671.1 dna:scaffold scaffold:CanFam3.1:AAEX03025671.1:1:5322:1 REF
      2329412539 5322 0
      0 AAEX03025670.1 dna:scaffold scaffold:CanFam3.1:AAEX03025670.1:1:5323:1 REF
      2329417861 5323 0
      0 AAEX03025669.1 dna:scaffold scaffold:CanFam3.1:AAEX03025669.1:1:5326:1 REF
      2329423184 5326 0
      0 AAEX03025668.1 dna:scaffold scaffold:CanFam3.1:AAEX03025668.1:1:5328:1 REF
      2329428510 5328 0
      0 AAEX03025667.1 dna:scaffold scaffold:CanFam3.1:AAEX03025667.1:1:5330:1 REF
      2329433838 5330 0
      0 AAEX03025666.1 dna:scaffold scaffold:CanFam3.1:AAEX03025666.1:1:5332:1 REF
      2329439168 5332 0
      0 AAEX03025665.1 dna:scaffold scaffold:CanFam3.1:AAEX03025665.1:1:5341:1 REF
      2329444500 5341 0
      0 AAEX03025663.1 dna:scaffold scaffold:CanFam3.1:AAEX03025663.1:1:5343:1 REF
      2329449841 5343 0
      0 AAEX03025664.1 dna:scaffold scaffold:CanFam3.1:AAEX03025664.1:1:5343:1 REF
      2329455184 5343 0
      0 AAEX03025662.1 dna:scaffold scaffold:CanFam3.1:AAEX03025662.1:1:5350:1 REF
      2329460527 5350 0
      0 AAEX03025661.1 dna:scaffold scaffold:CanFam3.1:AAEX03025661.1:1:5356:1 REF
      2329465877 5356 0
      0 AAEX03025660.1 dna:scaffold scaffold:CanFam3.1:AAEX03025660.1:1:5360:1 REF
      2329471233 5360 0
      0 AAEX03025659.1 dna:scaffold scaffold:CanFam3.1:AAEX03025659.1:1:5363:1 REF
      2329476593 5363 0
      0 AAEX03025658.1 dna:scaffold scaffold:CanFam3.1:AAEX03025658.1:1:5366:1 REF
      2329481956 5366 0
      0 AAEX03025657.1 dna:scaffold scaffold:CanFam3.1:AAEX03025657.1:1:5367:1 REF
      2329487322 5367 0
      0 AAEX03025656.1 dna:scaffold scaffold:CanFam3.1:AAEX03025656.1:1:5378:1 REF
      2329492689 5378 0
      0 AAEX03025655.1 dna:scaffold scaffold:CanFam3.1:AAEX03025655.1:1:5379:1 REF
      2329498067 5379 0
      0 AAEX03025654.1 dna:scaffold scaffold:CanFam3.1:AAEX03025654.1:1:5386:1 REF
      2329503446 5386 0
      0 AAEX03025653.1 dna:scaffold scaffold:CanFam3.1:AAEX03025653.1:1:5388:1 REF
      2329508832 5388 0
      0 AAEX03025652.1 dna:scaffold scaffold:CanFam3.1:AAEX03025652.1:1:5391:1 REF
      2329514220 5391 0
      0 AAEX03025649.1 dna:scaffold scaffold:CanFam3.1:AAEX03025649.1:1:5395:1 REF
      2329519611 5395 0
      0 AAEX03025647.1 dna:scaffold scaffold:CanFam3.1:AAEX03025647.1:1:5397:1 REF
      2329525006 5397 0
      0 AAEX03025648.1 dna:scaffold scaffold:CanFam3.1:AAEX03025648.1:1:5397:1 REF
      2329530403 5397 0
      0 AAEX03025646.1 dna:scaffold scaffold:CanFam3.1:AAEX03025646.1:1:5399:1 REF
      2329535800 5399 0
      0 AAEX03025644.1 dna:scaffold scaffold:CanFam3.1:AAEX03025644.1:1:5401:1 REF
      2329541199 5401 0
      0 AAEX03025645.1 dna:scaffold scaffold:CanFam3.1:AAEX03025645.1:1:5401:1 REF
      2329546600 5401 0
      0 AAEX03025643.1 dna:scaffold scaffold:CanFam3.1:AAEX03025643.1:1:5403:1 REF
      2329552001 5403 0
      0 AAEX03025642.1 dna:scaffold scaffold:CanFam3.1:AAEX03025642.1:1:5404:1 REF
      2329557404 5404 0
      0 AAEX03025641.1 dna:scaffold scaffold:CanFam3.1:AAEX03025641.1:1:5406:1 REF
      2329562808 5406 0
      0 AAEX03025640.1 dna:scaffold scaffold:CanFam3.1:AAEX03025640.1:1:5407:1 REF
      2329568214 5407 0
      0 AAEX03025639.1 dna:scaffold scaffold:CanFam3.1:AAEX03025639.1:1:5411:1 REF
      2329573621 5411 0
      0 AAEX03025638.1 dna:scaffold scaffold:CanFam3.1:AAEX03025638.1:1:5415:1 REF
      2329579032 5415 0
      0 AAEX03025637.1 dna:scaffold scaffold:CanFam3.1:AAEX03025637.1:1:5416:1 REF
      2329584447 5416 0
      0 AAEX03025636.1 dna:scaffold scaffold:CanFam3.1:AAEX03025636.1:1:5421:1 REF
      2329589863 5421 0
      0 AAEX03025635.1 dna:scaffold scaffold:CanFam3.1:AAEX03025635.1:1:5425:1 REF
      2329595284 5425 0
      0 AAEX03025634.1 dna:scaffold scaffold:CanFam3.1:AAEX03025634.1:1:5426:1 REF
      2329600709 5426 0
      0 AAEX03025633.1 dna:scaffold scaffold:CanFam3.1:AAEX03025633.1:1:5432:1 REF
      2329606135 5432 0
      0 AAEX03025632.1 dna:scaffold scaffold:CanFam3.1:AAEX03025632.1:1:5438:1 REF
      2329611567 5438 0
      0 AAEX03025631.1 dna:scaffold scaffold:CanFam3.1:AAEX03025631.1:1:5440:1 REF
      2329617005 5440 0
      0 AAEX03025629.1 dna:scaffold scaffold:CanFam3.1:AAEX03025629.1:1:5442:1 REF
      2329622445 5442 0
      0 AAEX03025630.1 dna:scaffold scaffold:CanFam3.1:AAEX03025630.1:1:5442:1 REF
      2329627887 5442 0
      0 AAEX03025628.1 dna:scaffold scaffold:CanFam3.1:AAEX03025628.1:1:5446:1 REF
      2329633329 5446 0
      0 AAEX03025627.1 dna:scaffold scaffold:CanFam3.1:AAEX03025627.1:1:5448:1 REF
      2329638775 5448 0
      0 AAEX03025623.1 dna:scaffold scaffold:CanFam3.1:AAEX03025623.1:1:5452:1 REF
      2329644223 5452 0
      0 AAEX03025624.1 dna:scaffold scaffold:CanFam3.1:AAEX03025624.1:1:5452:1 REF
      2329649675 5452 0
      0 AAEX03025622.1 dna:scaffold scaffold:CanFam3.1:AAEX03025622.1:1:5460:1 REF
      2329655127 5460 0
      0 AAEX03025621.1 dna:scaffold scaffold:CanFam3.1:AAEX03025621.1:1:5463:1 REF
      2329660587 5463 0
      0 AAEX03025620.1 dna:scaffold scaffold:CanFam3.1:AAEX03025620.1:1:5476:1 REF
      2329666050 5476 0
      0 AAEX03025619.1 dna:scaffold scaffold:CanFam3.1:AAEX03025619.1:1:5481:1 REF
      2329671526 5481 0
      0 AAEX03025618.1 dna:scaffold scaffold:CanFam3.1:AAEX03025618.1:1:5483:1 REF
      2329677007 5483 0
      0 AAEX03025617.1 dna:scaffold scaffold:CanFam3.1:AAEX03025617.1:1:5485:1 REF
      2329682490 5485 0
      0 AAEX03025615.1 dna:scaffold scaffold:CanFam3.1:AAEX03025615.1:1:5487:1 REF
      2329687975 5487 0
      0 AAEX03025616.1 dna:scaffold scaffold:CanFam3.1:AAEX03025616.1:1:5487:1 REF
      2329693462 5487 0
      0 AAEX03025614.1 dna:scaffold scaffold:CanFam3.1:AAEX03025614.1:1:5489:1 REF
      2329698949 5489 0
      0 AAEX03025613.1 dna:scaffold scaffold:CanFam3.1:AAEX03025613.1:1:5495:1 REF
      2329704438 5495 0
      0 AAEX03025612.1 dna:scaffold scaffold:CanFam3.1:AAEX03025612.1:1:5496:1 REF
      2329709933 5496 0
      0 AAEX03025611.1 dna:scaffold scaffold:CanFam3.1:AAEX03025611.1:1:5497:1 REF
      2329715429 5497 0
      0 AAEX03025610.1 dna:scaffold scaffold:CanFam3.1:AAEX03025610.1:1:5500:1 REF
      2329720926 5500 0
      0 AAEX03025609.1 dna:scaffold scaffold:CanFam3.1:AAEX03025609.1:1:5502:1 REF
      2329726426 5502 0
      0 AAEX03025608.1 dna:scaffold scaffold:CanFam3.1:AAEX03025608.1:1:5517:1 REF
      2329731928 5517 0
      0 AAEX03025607.1 dna:scaffold scaffold:CanFam3.1:AAEX03025607.1:1:5520:1 REF
      2329737445 5520 0
      0 AAEX03025606.1 dna:scaffold scaffold:CanFam3.1:AAEX03025606.1:1:5521:1 REF
      2329742965 5521 0
      0 AAEX03025605.1 dna:scaffold scaffold:CanFam3.1:AAEX03025605.1:1:5522:1 REF
      2329748486 5522 0
      0 AAEX03025604.1 dna:scaffold scaffold:CanFam3.1:AAEX03025604.1:1:5531:1 REF
      2329754008 5531 0
      0 AAEX03025602.1 dna:scaffold scaffold:CanFam3.1:AAEX03025602.1:1:5541:1 REF
      2329759539 5541 0
      0 AAEX03025603.1 dna:scaffold scaffold:CanFam3.1:AAEX03025603.1:1:5541:1 REF
      2329765080 5541 0
      0 AAEX03025601.1 dna:scaffold scaffold:CanFam3.1:AAEX03025601.1:1:5543:1 REF
      2329770621 5543 0
      0 AAEX03025600.1 dna:scaffold scaffold:CanFam3.1:AAEX03025600.1:1:5547:1 REF
      2329776164 5547 0
      0 AAEX03025599.1 dna:scaffold scaffold:CanFam3.1:AAEX03025599.1:1:5548:1 REF
      2329781711 5548 0
      0 AAEX03025598.1 dna:scaffold scaffold:CanFam3.1:AAEX03025598.1:1:5549:1 REF
      2329787259 5549 0
      0 AAEX03025596.1 dna:scaffold scaffold:CanFam3.1:AAEX03025596.1:1:5551:1 REF
      2329792808 5551 0
      0 AAEX03025597.1 dna:scaffold scaffold:CanFam3.1:AAEX03025597.1:1:5551:1 REF
      2329798359 5551 0
      0 AAEX03025595.1 dna:scaffold scaffold:CanFam3.1:AAEX03025595.1:1:5552:1 REF
      2329803910 5552 0
      0 AAEX03025594.1 dna:scaffold scaffold:CanFam3.1:AAEX03025594.1:1:5554:1 REF
      2329809462 5554 0
      0 AAEX03025593.1 dna:scaffold scaffold:CanFam3.1:AAEX03025593.1:1:5556:1 REF
      2329815016 5556 0
      0 AAEX03025592.1 dna:scaffold scaffold:CanFam3.1:AAEX03025592.1:1:5557:1 REF
      2329820572 5557 0
      0 AAEX03025591.1 dna:scaffold scaffold:CanFam3.1:AAEX03025591.1:1:5570:1 REF
      2329826129 5570 0
      0 AAEX03025590.1 dna:scaffold scaffold:CanFam3.1:AAEX03025590.1:1:5571:1 REF
      2329831699 5571 0
      0 AAEX03025588.1 dna:scaffold scaffold:CanFam3.1:AAEX03025588.1:1:5577:1 REF
      2329837270 5577 0
      0 AAEX03025589.1 dna:scaffold scaffold:CanFam3.1:AAEX03025589.1:1:5577:1 REF
      2329842847 5577 0
      0 AAEX03025587.1 dna:scaffold scaffold:CanFam3.1:AAEX03025587.1:1:5578:1 REF
      2329848424 5578 0
      0 AAEX03025586.1 dna:scaffold scaffold:CanFam3.1:AAEX03025586.1:1:5579:1 REF
      2329854002 5579 0
      0 AAEX03025585.1 dna:scaffold scaffold:CanFam3.1:AAEX03025585.1:1:5582:1 REF
      2329859581 5582 0
      0 AAEX03025584.1 dna:scaffold scaffold:CanFam3.1:AAEX03025584.1:1:5584:1 REF
      2329865163 5584 0
      0 AAEX03025583.1 dna:scaffold scaffold:CanFam3.1:AAEX03025583.1:1:5588:1 REF
      2329870747 5588 0
      0 AAEX03025582.1 dna:scaffold scaffold:CanFam3.1:AAEX03025582.1:1:5594:1 REF
      2329876335 5594 0
      0 AAEX03025581.1 dna:scaffold scaffold:CanFam3.1:AAEX03025581.1:1:5605:1 REF
      2329881929 5605 0
      0 AAEX03025580.1 dna:scaffold scaffold:CanFam3.1:AAEX03025580.1:1:5610:1 REF
      2329887534 5610 0
      0 AAEX03025578.1 dna:scaffold scaffold:CanFam3.1:AAEX03025578.1:1:5614:1 REF
      2329893144 5614 0
      0 AAEX03025579.1 dna:scaffold scaffold:CanFam3.1:AAEX03025579.1:1:5614:1 REF
      2329898758 5614 0
      0 AAEX03025577.1 dna:scaffold scaffold:CanFam3.1:AAEX03025577.1:1:5617:1 REF
      2329904372 5617 0
      0 AAEX03025576.1 dna:scaffold scaffold:CanFam3.1:AAEX03025576.1:1:5618:1 REF
      2329909989 5618 0
      0 AAEX03025575.1 dna:scaffold scaffold:CanFam3.1:AAEX03025575.1:1:5622:1 REF
      2329915607 5622 0
      0 AAEX03025574.1 dna:scaffold scaffold:CanFam3.1:AAEX03025574.1:1:5623:1 REF
      2329921229 5623 0
      0 AAEX03025573.1 dna:scaffold scaffold:CanFam3.1:AAEX03025573.1:1:5624:1 REF
      2329926852 5624 0
      0 AAEX03025572.1 dna:scaffold scaffold:CanFam3.1:AAEX03025572.1:1:5627:1 REF
      2329932476 5627 0
      0 AAEX03025570.1 dna:scaffold scaffold:CanFam3.1:AAEX03025570.1:1:5629:1 REF
      2329938103 5629 0
      0 AAEX03025571.1 dna:scaffold scaffold:CanFam3.1:AAEX03025571.1:1:5629:1 REF
      2329943732 5629 0
      0 AAEX03025569.1 dna:scaffold scaffold:CanFam3.1:AAEX03025569.1:1:5630:1 REF
      2329949361 5630 0
      0 AAEX03025567.1 dna:scaffold scaffold:CanFam3.1:AAEX03025567.1:1:5635:1 REF
      2329954991 5635 0
      0 AAEX03025568.1 dna:scaffold scaffold:CanFam3.1:AAEX03025568.1:1:5635:1 REF
      2329960626 5635 0
      0 AAEX03025566.1 dna:scaffold scaffold:CanFam3.1:AAEX03025566.1:1:5638:1 REF
      2329966261 5638 0
      0 AAEX03025565.1 dna:scaffold scaffold:CanFam3.1:AAEX03025565.1:1:5648:1 REF
      2329971899 5648 0
      0 AAEX03025564.1 dna:scaffold scaffold:CanFam3.1:AAEX03025564.1:1:5649:1 REF
      2329977547 5649 0
      0 AAEX03025563.1 dna:scaffold scaffold:CanFam3.1:AAEX03025563.1:1:5650:1 REF
      2329983196 5650 0
      0 AAEX03025562.1 dna:scaffold scaffold:CanFam3.1:AAEX03025562.1:1:5652:1 REF
      2329988846 5652 0
      0 AAEX03025561.1 dna:scaffold scaffold:CanFam3.1:AAEX03025561.1:1:5654:1 REF
      2329994498 5654 0
      0 AAEX03025560.1 dna:scaffold scaffold:CanFam3.1:AAEX03025560.1:1:5656:1 REF
      2330000152 5656 0
      0 AAEX03025559.1 dna:scaffold scaffold:CanFam3.1:AAEX03025559.1:1:5657:1 REF
      2330005808 5657 0
      0 AAEX03025558.1 dna:scaffold scaffold:CanFam3.1:AAEX03025558.1:1:5665:1 REF
      2330011465 5665 0
      0 AAEX03025557.1 dna:scaffold scaffold:CanFam3.1:AAEX03025557.1:1:5667:1 REF
      2330017130 5667 0
      0 AAEX03025556.1 dna:scaffold scaffold:CanFam3.1:AAEX03025556.1:1:5671:1 REF
      2330022797 5671 0
      0 AAEX03025555.1 dna:scaffold scaffold:CanFam3.1:AAEX03025555.1:1:5673:1 REF
      2330028468 5673 0
      0 AAEX03025553.1 dna:scaffold scaffold:CanFam3.1:AAEX03025553.1:1:5674:1 REF
      2330034141 5674 0
      0 AAEX03025554.1 dna:scaffold scaffold:CanFam3.1:AAEX03025554.1:1:5674:1 REF
      2330039815 5674 0
      0 AAEX03025552.1 dna:scaffold scaffold:CanFam3.1:AAEX03025552.1:1:5675:1 REF
      2330045489 5675 0
      0 AAEX03025551.1 dna:scaffold scaffold:CanFam3.1:AAEX03025551.1:1:5677:1 REF
      2330051164 5677 0
      0 AAEX03025550.1 dna:scaffold scaffold:CanFam3.1:AAEX03025550.1:1:5678:1 REF
      2330056841 5678 0
      0 AAEX03025549.1 dna:scaffold scaffold:CanFam3.1:AAEX03025549.1:1:5680:1 REF
      2330062519 5680 0
      0 AAEX03025548.1 dna:scaffold scaffold:CanFam3.1:AAEX03025548.1:1:5681:1 REF
      2330068199 5681 0
      0 AAEX03025547.1 dna:scaffold scaffold:CanFam3.1:AAEX03025547.1:1:5684:1 REF
      2330073880 5684 0
      0 AAEX03025544.1 dna:scaffold scaffold:CanFam3.1:AAEX03025544.1:1:5685:1 REF
      2330079564 5685 0
      0 AAEX03025543.1 dna:scaffold scaffold:CanFam3.1:AAEX03025543.1:1:5688:1 REF
      2330085249 5688 0
      0 AAEX03025542.1 dna:scaffold scaffold:CanFam3.1:AAEX03025542.1:1:5693:1 REF
      2330090937 5693 0
      0 AAEX03025541.1 dna:scaffold scaffold:CanFam3.1:AAEX03025541.1:1:5694:1 REF
      2330096630 5694 0
      0 AAEX03025540.1 dna:scaffold scaffold:CanFam3.1:AAEX03025540.1:1:5695:1 REF
      2330102324 5695 0
      0 AAEX03025539.1 dna:scaffold scaffold:CanFam3.1:AAEX03025539.1:1:5699:1 REF
      2330108019 5699 0
      0 JH374193.1 dna:scaffold scaffold:CanFam3.1:JH374193.1:1:5700:1 REF
      2330113718 5700 1
      0 AAEX03025538.1 dna:scaffold scaffold:CanFam3.1:AAEX03025538.1:1:5703:1 REF
      2330119418 5703 0
      0 AAEX03025537.1 dna:scaffold scaffold:CanFam3.1:AAEX03025537.1:1:5705:1 REF
      2330125121 5705 0
      0 AAEX03025536.1 dna:scaffold scaffold:CanFam3.1:AAEX03025536.1:1:5710:1 REF
      2330130826 5710 0
      0 AAEX03025532.1 dna:scaffold scaffold:CanFam3.1:AAEX03025532.1:1:5711:1 REF
      2330136536 5711 0
      0 AAEX03025531.1 dna:scaffold scaffold:CanFam3.1:AAEX03025531.1:1:5712:1 REF
      2330142247 5712 0
      0 AAEX03025530.1 dna:scaffold scaffold:CanFam3.1:AAEX03025530.1:1:5716:1 REF
      2330147959 5716 0
      0 AAEX03025529.1 dna:scaffold scaffold:CanFam3.1:AAEX03025529.1:1:5721:1 REF
      2330153675 5721 0
      0 AAEX03025528.1 dna:scaffold scaffold:CanFam3.1:AAEX03025528.1:1:5723:1 REF
      2330159396 5723 0
      0 AAEX03025527.1 dna:scaffold scaffold:CanFam3.1:AAEX03025527.1:1:5731:1 REF
      2330165119 5731 0
      0 AAEX03025526.1 dna:scaffold scaffold:CanFam3.1:AAEX03025526.1:1:5733:1 REF
      2330170850 5733 0
      0 AAEX03025525.1 dna:scaffold scaffold:CanFam3.1:AAEX03025525.1:1:5735:1 REF
      2330176583 5735 0
      0 AAEX03025524.1 dna:scaffold scaffold:CanFam3.1:AAEX03025524.1:1:5747:1 REF
      2330182318 5747 0
      0 AAEX03025523.1 dna:scaffold scaffold:CanFam3.1:AAEX03025523.1:1:5748:1 REF
      2330188065 5748 0

      Do you need more info?
      Again, I really appreciate your help!

      Comment

      • #4
        .ann looks alright seems (at least for the part showing here). Although .ann and .amb sizes are bigger than human genome.

        Do you still have the run info of ALN step both for the 330MB and 60MB output?

        Comment

        • #5
          1) Run info (one of the two reads): multifasta file generated from files from ensemble as ref genome sequence (60 MB output .sai file)

          bwa aln refGen3.fasta /Volumes/.....-DogCap-1_CGATGT_L003_R1_001.fastq.gz > aln_sa1.sai
          [bwa_aln] 17bp reads: max_diff = 2
          [bwa_aln] 38bp reads: max_diff = 3
          [bwa_aln] 64bp reads: max_diff = 4
          [bwa_aln] 93bp reads: max_diff = 5
          [bwa_aln] 124bp reads: max_diff = 6
          [bwa_aln] 157bp reads: max_diff = 7
          [bwa_aln] 190bp reads: max_diff = 8
          [bwa_aln] 225bp reads: max_diff = 9
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 262144 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.58 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 524288 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 786432 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 1048576 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 1310720 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 1572864 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 1835008 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 2097152 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 2359296 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.54 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 2621440 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.57 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 2883584 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 3145728 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 3407872 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 3670016 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 3932160 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 4194304 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 4456448 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 4718592 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.57 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 4980736 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 5242880 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.57 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 5505024 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 5767168 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 6029312 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 6291456 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 6553600 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 6815744 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 7077888 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.57 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 7340032 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 7602176 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 7864320 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 8126464 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 8388608 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 8650752 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 8912896 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.57 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 9175040 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 9437184 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 9699328 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.54 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 9961472 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 10223616 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 10485760 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 10747904 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 11010048 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.58 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 11272192 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 11534336 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 11796480 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 12058624 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 12320768 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.57 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 12582912 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 12845056 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 13107200 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 13369344 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 13631488 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.57 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 13893632 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 14155776 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.57 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 14417920 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.55 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 14680064 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 1.56 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 14942208 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 0.73 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 15064387 sequences have been processed.
          [main] Version: 0.6.2-r126
          [main] CMD: bwa aln refGen3.fasta /Volumes/.......-DogCap-1_CGATGT_L003_R1_001.fastq.gz
          [main] Real time: 188.896 sec; CPU: 156.373 sec

          2) Run info (one of the two reads): reference sequence = one fasta file of one chromosome from GenBank (330 MB output .sai file).

          bwa aln cf3.fasta /Volumes/...-DogCap-1/Dahlgren-DogCap-1_CGATGT_L003_R1_001.fastq.gz > aln_test1.sai
          [bwa_aln] 17bp reads: max_diff = 2
          [bwa_aln] 38bp reads: max_diff = 3
          [bwa_aln] 64bp reads: max_diff = 4
          [bwa_aln] 93bp reads: max_diff = 5
          [bwa_aln] 124bp reads: max_diff = 6
          [bwa_aln] 157bp reads: max_diff = 7
          [bwa_aln] 190bp reads: max_diff = 8
          [bwa_aln] 225bp reads: max_diff = 9
          [bwa_aln_core] calculate SA coordinate... 70.50 sec
          [bwa_aln_core] write to the disk... 0.04 sec
          [bwa_aln_core] 262144 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 70.45 sec
          [bwa_aln_core] write to the disk... 0.04 sec
          [bwa_aln_core] 524288 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 69.88 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 786432 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 67.47 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 1048576 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 66.83 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 1310720 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 68.28 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 1572864 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 68.40 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 1835008 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 68.76 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 2097152 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 68.45 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 2359296 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 65.06 sec
          [bwa_aln_core] write to the disk... 0.04 sec
          [bwa_aln_core] 2621440 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.91 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 2883584 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 61.75 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 3145728 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.32 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 3407872 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.70 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 3670016 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.13 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 3932160 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.35 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 4194304 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 64.04 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 4456448 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.24 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 4718592 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.05 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 4980736 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.17 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 5242880 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 61.90 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 5505024 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.63 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 5767168 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.43 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 6029312 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.92 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 6291456 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.55 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 6553600 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.43 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 6815744 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 64.90 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 7077888 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.37 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 7340032 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 61.23 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 7602176 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 60.06 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 7864320 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 60.83 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 8126464 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 64.54 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 8388608 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 66.63 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 8650752 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 66.04 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 8912896 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 66.94 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 9175040 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 65.03 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 9437184 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 64.13 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 9699328 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.01 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 9961472 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.21 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 10223616 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.42 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 10485760 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 61.12 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 10747904 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.63 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 11010048 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 61.57 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 11272192 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 64.17 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 11534336 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.45 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 11796480 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.50 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 12058624 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.00 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 12320768 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.32 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 12582912 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.61 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 12845056 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.90 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 13107200 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 60.26 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 13369344 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 61.79 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 13631488 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 63.02 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 13893632 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.08 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 14155776 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 61.49 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 14417920 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.19 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 14680064 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 62.14 sec
          [bwa_aln_core] write to the disk... 0.03 sec
          [bwa_aln_core] 14942208 sequences have been processed.
          [bwa_aln_core] calculate SA coordinate... 30.04 sec
          [bwa_aln_core] write to the disk... 0.01 sec
          [bwa_aln_core] 15064387 sequences have been processed.
          [main] Version: 0.6.2-r126
          [main] CMD: bwa aln cf3.fasta /Volumes/...-DogCap-1/Dahlgren-DogCap-1_CGATGT_L003_R1_001.fastq.gz
          [main] Real time: 3787.719 sec; CPU: 3740.718 sec

          Comment

          • #6
            Looks normal again.

            Now need to figure out if the .sai file is overwritten or everything inside is garbage. Can you cut 1 million rows from your fastq.gz (you can unzip first), run ALN, see how big the sai is, and then run SAMPE, see what SAM you can get.

            Comment

            • #7
              Have you tried running samse on the individual .sai files? Maybe it's hanging because the pairing is screwed up?

              Comment

              • #8
                So I cut 1 million rows from my fastq file and ran:

                1) bwa aln ...fastq, which generated a 1 MB .sai file (one for each direction).

                2) bwa sampe, which generates a 131.6 MB .sam file

                bwa sampe refGen3.fasta alnTestFirstOneMilrefGen3_1.sai alnTestFirstOneMilrefGen3_2.sai /...-DogCap-1_CGATGT_L003_R1_001.FirstOneMil.fastq /...-DogCap-1_CGATGT_L003_R2_001.FirstOneMil_R2.fastq > alnFirstOneMil.sam
                [bwa_sai2sam_pe_core] convert to sequence coordinate...
                [infer_isize] fail to infer insert size: too few good pairs
                [bwa_sai2sam_pe_core] time elapses: 2.21 sec
                [bwa_sai2sam_pe_core] changing coordinates of 0 alignments.
                [bwa_sai2sam_pe_core] align unmapped mate...
                [bwa_sai2sam_pe_core] time elapses: 0.63 sec
                [bwa_sai2sam_pe_core] refine gapped alignments... 0.87 sec
                [bwa_sai2sam_pe_core] print alignments... 0.86 sec
                [bwa_sai2sam_pe_core] 250000 sequences have been processed.
                [main] Version: 0.6.2-r126 refGen3.fasta alnTestFirstOneMilrefGen3_1.sai alnTestFirstOneMilrefGen3_2.sai /...-DogCap-1_CGATGT_L003_R1_001.FirstOneMil.fastq /...-DogCap-1_CGATGT_L003_R2_001.FirstOneMil_R2.fastq

                So why does sampe not work with the entire fastq files?

                Swbarnes: bwa sampe works fine when a use a fasta file from GenBank of only one chromosome, but not when I generate a whole genome file of all chromosomes (from ensemble). Do you still think it could be the pairing?

                Perhaps I should try samse as well.

                Many thanks for the advice i get.

                Comment

                • #9
                  In case you are still working today, 1 million reads to generate 1MB sai file, basically means each read only have a 0x00 there, i.e. not able to align at all, if you do hexdump, you might see lots of 0x00 all over the sai file (after a short header region).

                  Try: 1, when INDEX, give "-a bwtsw". (EDIT: no need to try this)
                  2, just cat chr1 + chr2, index, try with the 1million reads again.
                  Last edited by xied75; 12-03-2012, 08:53 AM. Reason: to correct mistake.

                  Comment

                  • #10
                    Hmm.. there is obviously something wrong with my whole-genome fasta file..

                    1, when INDEX, give "-a bwtsw". (EDIT: no need to try this)
                    I tried anyway, and as you said it did not work.

                    2, just cat chr1 + chr2, index, try with the 1million reads again.
                    I am not sure if this worked?
                    When I do bwa aln, the output files (.sai) are 5.9 MB (one for each direction). Probably too small?
                    And when I run bwa sampe, the output .sam file is 161.4 MB.
                    This is the sampe output:

                    [bwa_sai2sam_pe_core] convert to sequence coordinate...
                    [infer_isize] (25, 50, 75) percentile: (258, 290, 331)
                    [infer_isize] low and high boundaries: 112 and 477 for estimating avg and std
                    [infer_isize] inferred external isize from 127642 pairs: 293.914 +/- 58.128
                    [infer_isize] skewness: -0.004; kurtosis: 0.314; ap_prior: 1.36e-05
                    [infer_isize] inferred maximum insert size: 671 (6.49 sigma)
                    [bwa_sai2sam_pe_core] time elapses: 3.59 sec
                    [bwa_sai2sam_pe_core] changing coordinates of 1824 alignments.
                    [bwa_sai2sam_pe_core] align unmapped mate...
                    [bwa_paired_sw] 15515 out of 18826 Q17 singletons are mated.
                    [bwa_paired_sw] 1494 out of 2510 Q17 discordant pairs are fixed.
                    [bwa_sai2sam_pe_core] time elapses: 8.19 sec
                    [bwa_sai2sam_pe_core] refine gapped alignments... 1.10 sec
                    [bwa_sai2sam_pe_core] print alignments... 1.67 sec
                    [bwa_sai2sam_pe_core] 250000 sequences have been processed.
                    [main] Version: 0.6.2-r126

                    Comment

                    • #11
                      Originally posted by Stina View Post
                      Hmm.. there is obviously something wrong with my whole-genome fasta file..

                      1, when INDEX, give "-a bwtsw". (EDIT: no need to try this)
                      I tried anyway, and as you said it did not work.

                      2, just cat chr1 + chr2, index, try with the 1million reads again.
                      I am not sure if this worked?
                      When I do bwa aln, the output files (.sai) are 5.9 MB (one for each direction). Probably too small?
                      And when I run bwa sampe, the output .sam file is 161.4 MB.
                      This is the sampe output:

                      [bwa_sai2sam_pe_core] convert to sequence coordinate...
                      [infer_isize] (25, 50, 75) percentile: (258, 290, 331)
                      [infer_isize] low and high boundaries: 112 and 477 for estimating avg and std
                      [infer_isize] inferred external isize from 127642 pairs: 293.914 +/- 58.128
                      [infer_isize] skewness: -0.004; kurtosis: 0.314; ap_prior: 1.36e-05
                      [infer_isize] inferred maximum insert size: 671 (6.49 sigma)
                      [bwa_sai2sam_pe_core] time elapses: 3.59 sec
                      [bwa_sai2sam_pe_core] changing coordinates of 1824 alignments.
                      [bwa_sai2sam_pe_core] align unmapped mate...
                      [bwa_paired_sw] 15515 out of 18826 Q17 singletons are mated.
                      [bwa_paired_sw] 1494 out of 2510 Q17 discordant pairs are fixed.
                      [bwa_sai2sam_pe_core] time elapses: 8.19 sec
                      [bwa_sai2sam_pe_core] refine gapped alignments... 1.10 sec
                      [bwa_sai2sam_pe_core] print alignments... 1.67 sec
                      [bwa_sai2sam_pe_core] 250000 sequences have been processed.
                      [main] Version: 0.6.2-r126
                      Are you sure you are aligning a million reads? Because the bwa output looks like it is only aligning a quarter million

                      You realize that a fastq is 4 lines per read, right? So a fastq with a million lines only has a quarter million reads.

                      Comment

                      • #12
                        Are you sure you are aligning a million reads? Because the bwa output looks like it is only aligning a quarter million

                        You realize that a fastq is 4 lines per read, right? So a fastq with a million lines only has a quarter million reads.
                        Good catch!

                        If the library does have an insert size of around 290, then I think the problem is really to do with the whole ref fasta.

                        Comment

                        • #13
                          Thank you so much for trying to help me.

                          The problem was that I was using bwa version 6.2, which obviously doesnt work properly on a mac, see http://seqanswers.com/forums/showthread.php?t=24133

                          So I downloaded version 5.10 instead, which solved the problem.

                          Comment

                          Previous template Next

                          Latest Articles

                          Collapse
                          • seqadmin
                            Essential Discoveries and Tools in Epitranscriptomics
                            by seqadmin




                            The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
                            04-22-2024, 07:01 AM
                          • seqadmin
                            Current Approaches to Protein Sequencing
                            by seqadmin


                            Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
                            04-04-2024, 04:25 PM
                          View All

                          ad_right_rmr

                          Collapse

                          News

                          Collapse
                          Topics Statistics Last Post
                          Genetic Variants and Diabetes Risk in Childhood Cancer Survivors by seqadmin
                          Started by seqadmin, Today, 08:47 AM
                          0 responses
                          11 views
                          0 likes
                          		 Last Post seqadmin
                          by seqadmin
                          Today, 08:47 AM  
                          Cancer Metastasis: A Deep Dive into Cellular Plasticity by seqadmin
                          Started by seqadmin, 04-11-2024, 12:08 PM
                          0 responses
                          60 views
                          0 likes
                          		 Last Post seqadmin
                          by seqadmin
                          04-11-2024, 12:08 PM  
                          Proteogenomic Profiles Offer New Clues in Prostate Cancer by seqadmin
                          Started by seqadmin, 04-10-2024, 10:19 PM
                          0 responses
                          59 views
                          0 likes
                          		 Last Post seqadmin
                          by seqadmin
                          04-10-2024, 10:19 PM  
                          Novel Diagnostic Assay Enhances Ovarian Cancer Detection by seqadmin
                          Started by seqadmin, 04-10-2024, 09:21 AM
                          0 responses
                          54 views
                          0 likes
                          		 Last Post seqadmin
                          by seqadmin
                          04-10-2024, 09:21 AM  
                          View All
                          Working...
                          X