1. After emPCR step, then modified 3' end of the adapter to attached the glass slide, how to modified ? and which chemical reaction ?
2. To attached DNA to glass slide with covalent bond, how can the DNA fragment can be attached ? and the DNA under the bead ( DNA attached to glass slide )can be sequenced ?
3. How many DNA need to be attached to glass slide (to comfirm that the bead can be hold tighly with the glass slide) ?
Thank you so much for you information
2. To attached DNA to glass slide with covalent bond, how can the DNA fragment can be attached ? and the DNA under the bead ( DNA attached to glass slide )can be sequenced ?
3. How many DNA need to be attached to glass slide (to comfirm that the bead can be hold tighly with the glass slide) ?
Thank you so much for you information