Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Problem with DRAGEN Bio-IT Platform

    I have a problem with DRAGEN Bio-IT Platform.

    When I ran the map/alin and valiant caller pipeline, it stopped running by time out error.
    I asked to illumina technical support, and changed the usage memory amount using this option.

    " --bin_memory 60000000000 "

    However, this option and more usage memory (200,000,000,000) did not solve the problem, and error was printed as below.

    " The DRAGEN WatchDog service has detected a fault: Available free memory (1518 MB) is below minimum threshold (2048 MB) required by DRAGEN "

    I have enough memory server and I did not run any other scripts at the same when I ran DRAGEN pipeline.
    This probrem was happened when I input 193 Gbyte fastq.gz file.

    Could you tell me how I can solve this problem and/or the cause of this problem?

  • #2
    please send this to Illumina Tech-Support ( [email protected] )

    Comment


    • #3
      Hi did you got any solution? I am stuck in the same position and trying to figure out

      Comment


      • #4
        It has been a long time and I don't remember exactly, but I think I solved the problem by trimming the polyG at the end of reads.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Essential Discoveries and Tools in Epitranscriptomics
          by seqadmin




          The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
          Yesterday, 07:01 AM
        • seqadmin
          Current Approaches to Protein Sequencing
          by seqadmin


          Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
          04-04-2024, 04:25 PM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 04-11-2024, 12:08 PM
        0 responses
        58 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 10:19 PM
        0 responses
        54 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 09:21 AM
        0 responses
        45 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-04-2024, 09:00 AM
        0 responses
        55 views
        0 likes
        Last Post seqadmin  
        Working...
        X