Dear friends,
I have a small problem interpreting an indel that I found using exome sequencing with Illumina GA IIx.
When I look at the exome sequencing data it seems to be a clear heterozygote. We used conventional Sanger sequencing to confirm the indel but found it to be a clear homozygote. Apart from contamination and sequencing errors could there be some biological reason behind this? I have confirmed that the reads do not match to any other region in the genome.
I have a small problem interpreting an indel that I found using exome sequencing with Illumina GA IIx.
When I look at the exome sequencing data it seems to be a clear heterozygote. We used conventional Sanger sequencing to confirm the indel but found it to be a clear homozygote. Apart from contamination and sequencing errors could there be some biological reason behind this? I have confirmed that the reads do not match to any other region in the genome.
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