We have an Ion Proton that I will like to carry out fungal ITS amplicon sequencing on. I intend to use ITS1f and ITS 2 primers. However, while I have successfully carried out WGS on the Proton, this is the first time I will be doing amplicon seq. I do know I need to put in barcodes (as I have about 8 samples) and adapter. Would some one have a work flow I can modify or where I can get the necessary information? Is it possible to have custom made oligos with the adapter and barcodes? If so, how do you select your barcodes, their location on the oligos e.t.c?
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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