I have just set up my very first sequencing experiment on the Next-Seq 500. It is now 45 cycles into PE sequencing, however it seems that my QC scores are a bit low. I only have a % >= Q30%: 25.2%
I have around:
280 million reads in the QC 10 bin.
25 million in the QC 20 bin
8 million in the QC 25 bin
65 million in QC 30 bin
44 million in QC 35 bin.
Is this a bad result? Apologies for my stupidness, just was wondering whether data could be salvaged from this?
I have around:
280 million reads in the QC 10 bin.
25 million in the QC 20 bin
8 million in the QC 25 bin
65 million in QC 30 bin
44 million in QC 35 bin.
Is this a bad result? Apologies for my stupidness, just was wondering whether data could be salvaged from this?
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