I am trying to understand the difference between Agilent's Sureselect XT vs. XT2 exon captures.
From what I understand, with XT2, you pool all of the samples together and then use the capture. (V4 Exome + UTR) while with XT, you use the capture individually on each sample.
Is this understanding correct ?
Sorry if this question is too naive, I am mainly a bioinformatics person trying to understand the process.
From a Bioinformatics point of view, do you know if Agilent has separate BED files of targeted regions for XT and XT2 ? This is because I recently analyzed a set of samples, some of which were prepped using XT and others using XT2.
And for the ones with XT2, we did not find many reads mapping in the regions defined by Agilent's V4 + UTR BED file ? But there were a good amount of reads mapping in these regions for the XT samples.
Has anyone come across something like this before ?
From what I understand, with XT2, you pool all of the samples together and then use the capture. (V4 Exome + UTR) while with XT, you use the capture individually on each sample.
Is this understanding correct ?
Sorry if this question is too naive, I am mainly a bioinformatics person trying to understand the process.
From a Bioinformatics point of view, do you know if Agilent has separate BED files of targeted regions for XT and XT2 ? This is because I recently analyzed a set of samples, some of which were prepped using XT and others using XT2.
And for the ones with XT2, we did not find many reads mapping in the regions defined by Agilent's V4 + UTR BED file ? But there were a good amount of reads mapping in these regions for the XT samples.
Has anyone come across something like this before ?
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