Hello,
Last year we had a normalized cDNA library made by MWG and sequenced on 454 FLX with standard chemistry. One month later Titanium chemistry was released and we then learned that our very expensive normalized libraries could only be used with standard chemistry and never with Titanium. MWG should have told us this before hand!!! Anyway, I realise that the libraries fundamentally differ, but I am wondering if anyone knows of a homemade method to upgrade our libraries? Even if we lose some read length it would still be better than reverting to an earlier chemistry (we get 530 Mb with cDNA in Titanium and only got 115 Mb with standard chemistry).
Thanks!
Kathryn
Last year we had a normalized cDNA library made by MWG and sequenced on 454 FLX with standard chemistry. One month later Titanium chemistry was released and we then learned that our very expensive normalized libraries could only be used with standard chemistry and never with Titanium. MWG should have told us this before hand!!! Anyway, I realise that the libraries fundamentally differ, but I am wondering if anyone knows of a homemade method to upgrade our libraries? Even if we lose some read length it would still be better than reverting to an earlier chemistry (we get 530 Mb with cDNA in Titanium and only got 115 Mb with standard chemistry).
Thanks!
Kathryn
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