Hi Everyone,
I have done a number of TruSeq DNA preps, but now that it will be discontinued we have been looking into the TruSeq Nano prep. I have been reading the guide, but I found in the 'romoval of large DNA fragments' step things are confusing.
I see that 125ul needs to be transferred into a new plate (twice, thus that each plate will contain 250 ul)... ? So is this 1 plate with 250ul or two plates with each 125ul?
This continues with the removal of small fragments where 138ul and 276ul are also used as if it is the same? Really confusing and I don't see how this passed Illumina's check before it went out.
Did anyone come across this as well and how did you continue the prep?
With regards,
Dirk van essen
I have done a number of TruSeq DNA preps, but now that it will be discontinued we have been looking into the TruSeq Nano prep. I have been reading the guide, but I found in the 'romoval of large DNA fragments' step things are confusing.
I see that 125ul needs to be transferred into a new plate (twice, thus that each plate will contain 250 ul)... ? So is this 1 plate with 250ul or two plates with each 125ul?
This continues with the removal of small fragments where 138ul and 276ul are also used as if it is the same? Really confusing and I don't see how this passed Illumina's check before it went out.
Did anyone come across this as well and how did you continue the prep?
With regards,
Dirk van essen
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