Hello everyone,
I am using SAMTOOLS to view, sort, and index parts of a patient's DNA. But no matter what I do, I always get the same answer in alignment of this DNA with reference DNA: "empty."
I think the problem is that I don't have my data soft-clipped. How do I soft-clip data in samtools? Does samtools sort do soft-clipping? Because I already use samtools sort. Thanks!
I am using SAMTOOLS to view, sort, and index parts of a patient's DNA. But no matter what I do, I always get the same answer in alignment of this DNA with reference DNA: "empty."
I think the problem is that I don't have my data soft-clipped. How do I soft-clip data in samtools? Does samtools sort do soft-clipping? Because I already use samtools sort. Thanks!
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