Hi all,
We have a shotgun library prepared for 454 and would like to run it in ion torrent for extra data. We learned that the sequencing adaptor A is shared, but that the other adaptor is not (P1 for torrent, B for 454). The B adapter was, however, used in torrent some time ago and its sequence seems to be still present in the be bead, following P1. So the questions are:
- Should we convert by ligating just P1 adaptor?
- Any chance that we could run with no additional library conversion?
- Can an emulsion made with 454 primers and beads be loaded directly in torrent?
Any input will be most appreciated.
We have a shotgun library prepared for 454 and would like to run it in ion torrent for extra data. We learned that the sequencing adaptor A is shared, but that the other adaptor is not (P1 for torrent, B for 454). The B adapter was, however, used in torrent some time ago and its sequence seems to be still present in the be bead, following P1. So the questions are:
- Should we convert by ligating just P1 adaptor?
- Any chance that we could run with no additional library conversion?
- Can an emulsion made with 454 primers and beads be loaded directly in torrent?
Any input will be most appreciated.
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