Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Principle of chemical RNA Fragmentation

    Hello everyone,

    I'm Tobias, Research technician.

    I did some research regarding the the topic "Chemical RNA Fragmentation" and couldn't find anything helpfull.

    I mean, I'm facing problems with my NGS preparation regarding the fragment lenghts.
    If i have a very low amount of RNA (< 1ng input), my target reads (viral RNA) are likely to be ~200bp.
    Now I'm targeting Viral RNA within backround RNA of nearly 100ng and the fragmentation seems to be totally different.

    So - Specific Questions:

    - Does the ratio between MG2+ Ions and RNA fragments affect the fragmentation
    - How does MG2+ Ions and heat work on the RNA? Does the MG bind to the negative backbone, weaken the O-C connection between the bases? Or does the MG bin to the RNA, relaxing the strand and the movement of the RNA created by the heat simply breaks the RNA?
    - Does MG hate RNA and beat it up with tiny tiny baseball bats?

    I'm really looking forward to receive any hints and will be thankfull to receive answers

    With best regards

    Tobias

Latest Articles

Collapse

  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 06:37 PM
0 responses
11 views
0 likes
Last Post seqadmin  
Started by seqadmin, Yesterday, 06:07 PM
0 responses
10 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-22-2024, 10:03 AM
0 responses
51 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-21-2024, 07:32 AM
0 responses
67 views
0 likes
Last Post seqadmin  
Working...
X