SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
flowcell help seqgirl123 Illumina/Solexa 4 11-14-2013 04:49 AM
How to reduce flowcell bubbles in the HiSeq? pmiguel Illumina/Solexa 20 04-09-2013 11:17 AM
PolyA on flowcell biochembug Illumina/Solexa 1 09-22-2011 09:05 AM
EZ bead Bubbles after transfering emulsion asaleh SOLiD 1 06-20-2011 05:32 AM
Bubbles and XD slides pmiguel SOLiD 0 10-13-2010 05:54 AM

Reply
 
Thread Tools
Old 07-13-2014, 09:37 AM   #1
DRYTCYV
Member
 
Location: Portugal

Join Date: Apr 2011
Posts: 50
Default Bubbles in Miseq Flowcell

Hi everyone,

I would like to know your opinion about the images in attachment.
The images are from different Runs, but this is the 5th Run that we detected strange things like this bubble.
I don’t know if you check the images in every Run, but for you, what kind of strange things you find in the images and assume that its normal?

Thanks

https://www.dropbox.com/s/32pva4lnde0zim4/Run%23C.png
https://www.dropbox.com/s/gvwxxr9rsg8yagc/Run%23B.png
https://www.dropbox.com/sc/er5vt25tb...e8-7hMm_EWELqa

Last edited by DRYTCYV; 07-13-2014 at 09:47 AM.
DRYTCYV is offline   Reply With Quote
Old 07-14-2014, 12:03 AM   #2
dfhdfh
Member
 
Location: Germany

Join Date: Jan 2013
Posts: 46
Default

I observed similar things as well. These runs more or less failed with bad clustering efficiency. Illumina support is on the case but hasn't solved anything, yet (and I don't know whether the bubbles are the cause).
dfhdfh is offline   Reply With Quote
Old 07-15-2014, 08:15 AM   #3
DRYTCYV
Member
 
Location: Portugal

Join Date: Apr 2011
Posts: 50
Default

Hi dfhdfh,

Thank you for your feedback.
We alreday runned the Miseq test and there's no problem in the fluidic system, but our local support will be here tomorrow to change some part from the fluidic system.

No more feedback? anyone?
DRYTCYV is offline   Reply With Quote
Old 07-18-2014, 06:07 PM   #4
snetmcom
Senior Member
 
Location: USA

Join Date: Oct 2008
Posts: 156
Default

Bubbles are not that uncommon. It really depends on the frequency. I'd be curious if you find any tips.
snetmcom is offline   Reply With Quote
Reply

Tags
miseq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 06:44 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO