I would like to check the integrity of crude DNA and RNA samples extracted from tissues in order to know if it is worth keep going with library prep and sequencing (MiSeq or HiSeq).
(1) Is running the crude DNA extracts on BioAnalyzer informative to know if the samples are sequence-able?
(I know for RNA I could use the RIN values)
(2) If yes, what particular criteria should be taken into consideration (presence of sharp bands/smear in the gel; broad/sharp peaks on the electropherogram)?
(3) Any other possible approach than Bioanalyzer?
Thanks !
(1) Is running the crude DNA extracts on BioAnalyzer informative to know if the samples are sequence-able?
(I know for RNA I could use the RIN values)
(2) If yes, what particular criteria should be taken into consideration (presence of sharp bands/smear in the gel; broad/sharp peaks on the electropherogram)?
(3) Any other possible approach than Bioanalyzer?
Thanks !
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