Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Cufflinks stuck at assembling transcript and initializong abundances step Him26 Bioinformatics 1 12-21-2012 11:41 AM
TruSeq Custom Amplicon - Extension-Ligation Step brachysclereid Sample Prep / Library Generation 1 12-18-2012 01:16 PM
Tophat 2.0.0 is stuck EGrassi Bioinformatics 8 10-24-2012 03:08 AM
TruSeq RNA-How many samples to pool? mchotalia Sample Prep / Library Generation 0 01-11-2012 06:27 AM
step by step for rarefaction calculation psong Metagenomics 1 01-06-2010 05:08 AM

Thread Tools
Old 03-01-2013, 08:18 AM   #1
Junior Member
Location: Zürich

Join Date: Sep 2011
Posts: 4
Default stuck in normalize and pool step TruSeq


Well today I feel accomplished because I manage to almost finish my library prep successfully. Now the next step got me thinking. Normalize and pool. I run the bioanalyzer 2100 and I wanted to start making the calculations for normalizing. Is there a unique value -as in the bioanalyser results- for normalizing each library? there is a list of the peaks with its own molarity value, should I just make an average of them? should I just add them? I know the answer must be some very basic stuff, but I would really appreciate your help,

all the best
anamar is offline   Reply With Quote

bioanalyzer, molarity, normalisation, pooling, truseq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 06:18 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO