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Old 02-26-2013, 05:39 AM   #1
MesutOezil
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Location: Japan

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Arrow mid-range inserts for HiSeq de novo genome sequencing

Hello. I wonder if any of you is familiar with the protocol to generate paired-end DNA library for illumina HiSeq in de novo genome sequencing.

I did paired-end sequencing with 200bp inserts, and want to try with longer inserts (e.g., 800bp, 2kb) to get higher continuity of assembly.

At PAG meeting in San Diego early this year, I heard about a series of libraries with different insert lengths used in the Parrot genome project, but have not found any publication and technical information for that project.
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Old 02-26-2013, 06:31 AM   #2
TonyBrooks
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http://core-genomics.blogspot.co.uk/...-possible.html

I would also check out this post, where long inserts have been shown to still sequencing. This indicates that 800bp inserts should be fine (certainly on MiSeq).
http://seqanswers.com/forums/showthread.php?t=20839
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