Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
Convert Cytogenetic location to base pair position LouiseT Bioinformatics 0 03-04-2013 07:18 PM
Extracting the base/nucleotide across all reads at particular position/s empyrean Bioinformatics 8 10-02-2012 08:21 AM
Nucleotide Distribution per Ref Base Software in BAM? twaddlac Bioinformatics 0 06-21-2012 06:45 AM
A basic base pair (dna) question ardmore General 12 08-24-2011 10:10 AM

Thread Tools
Old 01-14-2019, 05:42 PM   #1
Junior Member
Location: Malaysia

Join Date: Apr 2018
Posts: 4
Default nucleotide versus base pair

Hello everyone

I have a basic question to ask.
What is the difference between nucleotide and base pair?

From what I understand, nucleotide are A, C, G and T.
Base pair is the nucleotide with its complimentary nucleotide, (A-T or vice versa and G-C or vice versa).

If Im doing DNA Sequencing using FASTA,

QUERY Sequence.txt:


LIBRARY Sequence.txt:


so the QUERY Sequence.txt contain 8bp or 8 nucleoties?

Also i found some article would write 25kb.
Does kb means kilo bytes or kilo base pair?
If kilo bytes, does this determine by the saved file size?

Thank you very much.
dayana42200 is offline   Reply With Quote
Old 01-15-2019, 07:13 AM   #2
Location: Oklahoma

Join Date: Jan 2012
Posts: 35

I use them interchangeably. If you have 8 nucleotides you automatically have 8 bp. Technically, of course, they are different.
fznajar is offline   Reply With Quote
Old 01-16-2019, 08:02 PM   #3
Junior Member
Location: Toronto, ON, Canada

Join Date: Jul 2018
Posts: 3

Your understanding is correct - a nucleotide is an individual base, a base pair refers to the base and its complement.
I'm with fznajar; they're used pretty much interchangeably, despite having different meanings. Sometimes the distinction is relevant, often it isn't.

In your example the query sequence is 8 nucelotides (8 bases).

Also i found some article would write 25kb
The article means 25 kilo bases - 25000 bases. Generally speaking, people will talk about DNA in its single-stranded form, particularly when talking about lengths. We know of course that each of the bases has a complementary base, meaning that there are also 25 kilobase pairs, but it's less ambiguous to talk about bases (how many total bases/nucleotides are there in 25 kilobase pairs -- 25000? 50000? two different readers may come to different conclusions).
hkeward is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 11:58 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO