SEQanswers nucleotide versus base pair
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 01-14-2019, 05:42 PM #1 dayana42200 Junior Member   Location: Malaysia Join Date: Apr 2018 Posts: 4 nucleotide versus base pair Hello everyone I have a basic question to ask. What is the difference between nucleotide and base pair? From what I understand, nucleotide are A, C, G and T. Base pair is the nucleotide with its complimentary nucleotide, (A-T or vice versa and G-C or vice versa). If Im doing DNA Sequencing using FASTA, QUERY Sequence.txt: >1 AGGTCATT LIBRARY Sequence.txt: >Dataset ACGTGTAT so the QUERY Sequence.txt contain 8bp or 8 nucleoties? Also i found some article would write 25kb. Does kb means kilo bytes or kilo base pair? If kilo bytes, does this determine by the saved file size? Thank you very much.
 01-15-2019, 07:13 AM #2 fznajar Member   Location: Oklahoma Join Date: Jan 2012 Posts: 35 I use them interchangeably. If you have 8 nucleotides you automatically have 8 bp. Technically, of course, they are different.
01-16-2019, 08:02 PM   #3
hkeward
Junior Member

Location: Toronto, ON, Canada

Join Date: Jul 2018
Posts: 3

Your understanding is correct - a nucleotide is an individual base, a base pair refers to the base and its complement.
I'm with fznajar; they're used pretty much interchangeably, despite having different meanings. Sometimes the distinction is relevant, often it isn't.

In your example the query sequence is 8 nucelotides (8 bases).

Quote:
 Also i found some article would write 25kb
The article means 25 kilo bases - 25000 bases. Generally speaking, people will talk about DNA in its single-stranded form, particularly when talking about lengths. We know of course that each of the bases has a complementary base, meaning that there are also 25 kilobase pairs, but it's less ambiguous to talk about bases (how many total bases/nucleotides are there in 25 kilobase pairs -- 25000? 50000? two different readers may come to different conclusions).