Hi,
I was wondering if anyone has experience of using less than 1ug of input DNA for the TruSeq DNA library prep? I am assuming i would have to alter the covaris shearing settings and also dilute the adapters in the ligation step to avoid adapter dimers? Does anyone have tips for this?
In addition, i recently made TruSeq DNA libraries using 1ug of DNA and followed the instructions carefully. I ended up with so much library that i was thinking of next time quantifying the library after size selection and if there is enough then i would omit the enrichment step. Is there any reason why i should not do this?
It'll be great to hear your thoughts.
Thanks in advance.
I was wondering if anyone has experience of using less than 1ug of input DNA for the TruSeq DNA library prep? I am assuming i would have to alter the covaris shearing settings and also dilute the adapters in the ligation step to avoid adapter dimers? Does anyone have tips for this?
In addition, i recently made TruSeq DNA libraries using 1ug of DNA and followed the instructions carefully. I ended up with so much library that i was thinking of next time quantifying the library after size selection and if there is enough then i would omit the enrichment step. Is there any reason why i should not do this?
It'll be great to hear your thoughts.
Thanks in advance.
Comment