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Does small RNA sequencing generate comparable reads as regular Solexa sequencing? Goodluck Illumina/Solexa 1 08-19-2009 02:12 AM

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Old 02-01-2018, 10:23 PM   #1
fuellen
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Location: Germany

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Default RNA Sequencing at doctor's office

Hello!
I'm looking for feasible scenarios that would enable gene expression data analyses at a doctor's office, probably based on a blood sample. It seems that sample processing is too sophisticated to be done there, so samples would have to be frozen and shipped. Question: How long can blood samples, after minimal processing, be stored at -18 degrees Celsius (or at +4 degrees, possibly) so that they may then be sent in bulk (eg on dry ice) to a sequencing facility?
Thanks for any hints!
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Old 02-02-2018, 05:31 AM   #2
HESmith
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Expression profiles can change in minutes, so I'd recommend adding a stabilization component (e.g., Qiagen's RNAprotect) immediately after collection. At that point, the samples would be stable for weeks at 4˚ and indefinitely if frozen.
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Old 02-09-2018, 07:43 AM   #3
GW_OK
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Is there a reason you can't draw into a PAXgene tube?
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