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Old 03-02-2018, 04:56 AM   #1
Junior Member
Location: The Netherlands

Join Date: May 2017
Posts: 8
Default Overloading QiaAmp minelute virus spin columns

did anyone try to include more volume than recommended 200uL on QiaAmp minelute virus spin kit columns ? As for exapmle just loading the lysed sample in two rounds on the column to bind? I want to load 400uL, human cells.
Any tips/suggestions? Did it have very negative impact on your yield?

(I know there are different kits, also QiaAmp vacuum, but I would really like to use the one mentioned above)
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